Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations

Frederick A. Bettelheim, A. Coolidge Churchill, W. Gerald Robison, J. Samuel Zigler

Research output: Contribution to journalArticle

Abstract

Rat lenses incubated in dimethyl sulfoxide (DMSO)-water binary mixtures of different compositions became turbid. A slight haziness developed up to 0.062 mole fraction of DMSO; at higher concentrations dense turbidity developed. Microscopic examination of the incubated rat lenses showed damage restricted to the epithelium and outer cortex at low DMSO concentrations; maximum damage occurred, in terms of fiber cell swelling, extracellular fluid (lake) formation and disintegration of epithelium, at 0.25 mole ratio of DMSO. Scanning electron microscopic observation showed that at high DMSO concentrations, especially at 0.25 mole fraction, the contents of the fiber cells were greatly damaged. Apparently a large part of the crystallins had coalesced around the cytoskeletal bodies. Polarized light-scattering intensity measurements and their analyses indicated that when dense opacities developed at high DMSO concentrations, the major contribution to the turbidity came from the optical anisotropy fluctuations. The change in the organization of the components within the fiber cells disturbs the balance between intrinsic birefringence and form birefringence necessary for transparency. Thus, the DMSO-caused opacification can be described as a good model for the involvement of optical anisotropy fluctuations in Cataractoenesis.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalJournal of Biomedical Optics
Volume1
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

cataracts
Optical anisotropy
Dimethyl sulfoxide
Anisotropy
Dimethyl Sulfoxide
Cataract
anisotropy
epithelium
turbidity
rats
fibers
birefringence
Birefringence
cells
lenses
damage
Turbidity
cortexes
disintegration
Lenses

Keywords

  • Cataract
  • Dimethyl sulfoxide
  • Light scatter and fluorescence
  • Optical anisotrophy
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Atomic and Molecular Physics, and Optics
  • Radiological and Ultrasound Technology

Cite this

Bettelheim, F. A., Churchill, A. C., Robison, W. G., & Zigler, J. S. (1996). Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations. Journal of Biomedical Optics, 1(3), 273-279.

Dimethyl sulfoxide cataract : a model for optical anisotropy fluctuations. / Bettelheim, Frederick A.; Churchill, A. Coolidge; Robison, W. Gerald; Zigler, J. Samuel.

In: Journal of Biomedical Optics, Vol. 1, No. 3, 1996, p. 273-279.

Research output: Contribution to journalArticle

Bettelheim, FA, Churchill, AC, Robison, WG & Zigler, JS 1996, 'Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations', Journal of Biomedical Optics, vol. 1, no. 3, pp. 273-279.
Bettelheim FA, Churchill AC, Robison WG, Zigler JS. Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations. Journal of Biomedical Optics. 1996;1(3):273-279.
Bettelheim, Frederick A. ; Churchill, A. Coolidge ; Robison, W. Gerald ; Zigler, J. Samuel. / Dimethyl sulfoxide cataract : a model for optical anisotropy fluctuations. In: Journal of Biomedical Optics. 1996 ; Vol. 1, No. 3. pp. 273-279.
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