Dimerization of the tat protein from human immunodeficiency virus: A cysteine-rich peptide mimics the normal metal-linked dimer interface

A. D. Frankel, L. Chen, R. J. Cotter, C. O. Pabo

Research output: Contribution to journalArticlepeer-review

Abstract

We have synthesized an 18-amino acid peptide that contains the cysteine-rich region of the tat protein from human immunodeficiency virus. Previous experiments in vitro with the intact tat protein have shown that these cysteines serve as metal ligands, causing tat to form metal-linked dimers. Ultraviolet absorption spectra show that the synthetic peptide (tat21-38) binds two Cd2+ or two Zn2+ ions per peptide monomer, and some changes in the circular dichroism spectra are seen as the metals bind. The peptide-metal complexes are completely resistant to proteolytic digestion, and mass spectrometry demonstrates that this peptide forms metal-linked dimers. The peptide can also combine with the intact tat protein to form metal-linked heterodimers. If these heterodimers are unable to trans-activate viral transcription, tat21-38 could be a lead compound for designing drugs to treat acquired immunodeficiency syndrome.

Original languageEnglish (US)
Pages (from-to)6297-6300
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number17
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • General

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