Diffusional mobility of Golgi proteins in membranes of living cells

Nelson B. Cole, Carolyn L. Smith, Noah Sciaky, Mark Terasaki, Michael A Edidin, Jennifer Lippincott-Schwartz

Research output: Contribution to journalArticle

Abstract

The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 X 10-9 square centimeters per second to 5 x 10-9 square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.

Original languageEnglish (US)
Pages (from-to)797-801
Number of pages5
JournalScience
Volume273
Issue number5276
StatePublished - 1996

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Membrane Proteins
Cell Membrane
Proteins
Fluorescence Recovery After Photobleaching
Golgi Apparatus
HeLa Cells
Immobilization
Lipids
Membranes

ASJC Scopus subject areas

  • General

Cite this

Cole, N. B., Smith, C. L., Sciaky, N., Terasaki, M., Edidin, M. A., & Lippincott-Schwartz, J. (1996). Diffusional mobility of Golgi proteins in membranes of living cells. Science, 273(5276), 797-801.

Diffusional mobility of Golgi proteins in membranes of living cells. / Cole, Nelson B.; Smith, Carolyn L.; Sciaky, Noah; Terasaki, Mark; Edidin, Michael A; Lippincott-Schwartz, Jennifer.

In: Science, Vol. 273, No. 5276, 1996, p. 797-801.

Research output: Contribution to journalArticle

Cole, NB, Smith, CL, Sciaky, N, Terasaki, M, Edidin, MA & Lippincott-Schwartz, J 1996, 'Diffusional mobility of Golgi proteins in membranes of living cells', Science, vol. 273, no. 5276, pp. 797-801.
Cole NB, Smith CL, Sciaky N, Terasaki M, Edidin MA, Lippincott-Schwartz J. Diffusional mobility of Golgi proteins in membranes of living cells. Science. 1996;273(5276):797-801.
Cole, Nelson B. ; Smith, Carolyn L. ; Sciaky, Noah ; Terasaki, Mark ; Edidin, Michael A ; Lippincott-Schwartz, Jennifer. / Diffusional mobility of Golgi proteins in membranes of living cells. In: Science. 1996 ; Vol. 273, No. 5276. pp. 797-801.
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