Diffusion-weighted and Gd-EOB-DTPA-contrast-enhanced magnetic resonance imaging for characterization of tumor necrosis in an animal model

Josephina A. Vossen, Manon Buijs, Jean Francois H Geschwind, Eleni A Liapi, Veronica Prieto Ventura, Kwang Hun Lee, David A. Bluemke, Ihab R Kamel

Research output: Contribution to journalArticle

Abstract

Purpose:: To evaluate the role of diffusion-weighted magnetic resonance imaging (MRI) in determining tumor necrosis and contrast-enhanced MRI using gadoxetic acid disodium (Gd-EOB-DTPA) in determining maximum tumor size measurement and tumor delineation compared with criterion-standard histologic measurements in the rabbit VX2 liver tumor model. Materials and Methods:: VX2 tumors were implanted in the livers of 13 rabbits. Magnetic resonance imaging was performed using a 1.5-T MRI scanner and an extremity coil. The imaging protocol included T2-weighted fast spin-echo images, 3-dimensional T1-weighted spoiled gradient-echo with and without fat suppression after administration of Gd-EOB-DTPA, and diffusion-weighted echo planar images. Rabbits were killed, and the tumor was harvested and sliced at 4-mm intervals in the axial plane. The MRI parameters evaluated were tumor size, tumor delineation, and tumor apparent diffusion coefficient (ADC) values. Histologic sections were evaluated to quantify tumor necrosis. Results:: On contrast-enhanced MRI (obtained from 11 rabbits), the mean tumor sizes were 20, 19, and 20 mm in the arterial, portal venous, and delayed phases, respectively. Tumor delineation was most distinguishable in the delayed phase. On diffusion-weighted MRI (acquired in 13 rabbits), the mean tumor ADC value was 1.84 × 10 mm/s. The mean tumor size at pathology was 16 mm. The mean percent necrosis at the tumor's pathologic condition was 36%. The correlation between ADC value and percent necrosis showed an R value of 0.68. Conclusions:: Contrast-enhanced MRI using Gd-EOB-DTPA may provide additional information about tumor outline in the liver. Moreover, we showed a remarkable correlation between ADC values and tumor necrosis. Thus, diffusion-weighted imaging may be useful to assess tumor necrosis; nevertheless, the search for new modalities remains important.

Original languageEnglish (US)
Pages (from-to)626-630
Number of pages5
JournalJournal of Computer Assisted Tomography
Volume33
Issue number4
DOIs
StatePublished - Jul 2009

Fingerprint

Necrosis
Animal Models
Magnetic Resonance Imaging
Neoplasms
Rabbits
gadolinium ethoxybenzyl DTPA
Diffusion Magnetic Resonance Imaging
Liver
Extremities
Fats

Keywords

  • Diffusion-weighted MRI
  • Gd-EOB-DTPA
  • HCC
  • Liver cancer
  • Tumor necrosis
  • Tumor size
  • VX2

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Diffusion-weighted and Gd-EOB-DTPA-contrast-enhanced magnetic resonance imaging for characterization of tumor necrosis in an animal model. / Vossen, Josephina A.; Buijs, Manon; Geschwind, Jean Francois H; Liapi, Eleni A; Prieto Ventura, Veronica; Lee, Kwang Hun; Bluemke, David A.; Kamel, Ihab R.

In: Journal of Computer Assisted Tomography, Vol. 33, No. 4, 07.2009, p. 626-630.

Research output: Contribution to journalArticle

Vossen, Josephina A. ; Buijs, Manon ; Geschwind, Jean Francois H ; Liapi, Eleni A ; Prieto Ventura, Veronica ; Lee, Kwang Hun ; Bluemke, David A. ; Kamel, Ihab R. / Diffusion-weighted and Gd-EOB-DTPA-contrast-enhanced magnetic resonance imaging for characterization of tumor necrosis in an animal model. In: Journal of Computer Assisted Tomography. 2009 ; Vol. 33, No. 4. pp. 626-630.
@article{a90ca420aa5442fab506095796ce90e4,
title = "Diffusion-weighted and Gd-EOB-DTPA-contrast-enhanced magnetic resonance imaging for characterization of tumor necrosis in an animal model",
abstract = "Purpose:: To evaluate the role of diffusion-weighted magnetic resonance imaging (MRI) in determining tumor necrosis and contrast-enhanced MRI using gadoxetic acid disodium (Gd-EOB-DTPA) in determining maximum tumor size measurement and tumor delineation compared with criterion-standard histologic measurements in the rabbit VX2 liver tumor model. Materials and Methods:: VX2 tumors were implanted in the livers of 13 rabbits. Magnetic resonance imaging was performed using a 1.5-T MRI scanner and an extremity coil. The imaging protocol included T2-weighted fast spin-echo images, 3-dimensional T1-weighted spoiled gradient-echo with and without fat suppression after administration of Gd-EOB-DTPA, and diffusion-weighted echo planar images. Rabbits were killed, and the tumor was harvested and sliced at 4-mm intervals in the axial plane. The MRI parameters evaluated were tumor size, tumor delineation, and tumor apparent diffusion coefficient (ADC) values. Histologic sections were evaluated to quantify tumor necrosis. Results:: On contrast-enhanced MRI (obtained from 11 rabbits), the mean tumor sizes were 20, 19, and 20 mm in the arterial, portal venous, and delayed phases, respectively. Tumor delineation was most distinguishable in the delayed phase. On diffusion-weighted MRI (acquired in 13 rabbits), the mean tumor ADC value was 1.84 × 10 mm/s. The mean tumor size at pathology was 16 mm. The mean percent necrosis at the tumor's pathologic condition was 36{\%}. The correlation between ADC value and percent necrosis showed an R value of 0.68. Conclusions:: Contrast-enhanced MRI using Gd-EOB-DTPA may provide additional information about tumor outline in the liver. Moreover, we showed a remarkable correlation between ADC values and tumor necrosis. Thus, diffusion-weighted imaging may be useful to assess tumor necrosis; nevertheless, the search for new modalities remains important.",
keywords = "Diffusion-weighted MRI, Gd-EOB-DTPA, HCC, Liver cancer, Tumor necrosis, Tumor size, VX2",
author = "Vossen, {Josephina A.} and Manon Buijs and Geschwind, {Jean Francois H} and Liapi, {Eleni A} and {Prieto Ventura}, Veronica and Lee, {Kwang Hun} and Bluemke, {David A.} and Kamel, {Ihab R}",
year = "2009",
month = "7",
doi = "10.1097/RCT.0b013e3181953df3",
language = "English (US)",
volume = "33",
pages = "626--630",
journal = "Journal of Computer Assisted Tomography",
issn = "0363-8715",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Diffusion-weighted and Gd-EOB-DTPA-contrast-enhanced magnetic resonance imaging for characterization of tumor necrosis in an animal model

AU - Vossen, Josephina A.

AU - Buijs, Manon

AU - Geschwind, Jean Francois H

AU - Liapi, Eleni A

AU - Prieto Ventura, Veronica

AU - Lee, Kwang Hun

AU - Bluemke, David A.

AU - Kamel, Ihab R

PY - 2009/7

Y1 - 2009/7

N2 - Purpose:: To evaluate the role of diffusion-weighted magnetic resonance imaging (MRI) in determining tumor necrosis and contrast-enhanced MRI using gadoxetic acid disodium (Gd-EOB-DTPA) in determining maximum tumor size measurement and tumor delineation compared with criterion-standard histologic measurements in the rabbit VX2 liver tumor model. Materials and Methods:: VX2 tumors were implanted in the livers of 13 rabbits. Magnetic resonance imaging was performed using a 1.5-T MRI scanner and an extremity coil. The imaging protocol included T2-weighted fast spin-echo images, 3-dimensional T1-weighted spoiled gradient-echo with and without fat suppression after administration of Gd-EOB-DTPA, and diffusion-weighted echo planar images. Rabbits were killed, and the tumor was harvested and sliced at 4-mm intervals in the axial plane. The MRI parameters evaluated were tumor size, tumor delineation, and tumor apparent diffusion coefficient (ADC) values. Histologic sections were evaluated to quantify tumor necrosis. Results:: On contrast-enhanced MRI (obtained from 11 rabbits), the mean tumor sizes were 20, 19, and 20 mm in the arterial, portal venous, and delayed phases, respectively. Tumor delineation was most distinguishable in the delayed phase. On diffusion-weighted MRI (acquired in 13 rabbits), the mean tumor ADC value was 1.84 × 10 mm/s. The mean tumor size at pathology was 16 mm. The mean percent necrosis at the tumor's pathologic condition was 36%. The correlation between ADC value and percent necrosis showed an R value of 0.68. Conclusions:: Contrast-enhanced MRI using Gd-EOB-DTPA may provide additional information about tumor outline in the liver. Moreover, we showed a remarkable correlation between ADC values and tumor necrosis. Thus, diffusion-weighted imaging may be useful to assess tumor necrosis; nevertheless, the search for new modalities remains important.

AB - Purpose:: To evaluate the role of diffusion-weighted magnetic resonance imaging (MRI) in determining tumor necrosis and contrast-enhanced MRI using gadoxetic acid disodium (Gd-EOB-DTPA) in determining maximum tumor size measurement and tumor delineation compared with criterion-standard histologic measurements in the rabbit VX2 liver tumor model. Materials and Methods:: VX2 tumors were implanted in the livers of 13 rabbits. Magnetic resonance imaging was performed using a 1.5-T MRI scanner and an extremity coil. The imaging protocol included T2-weighted fast spin-echo images, 3-dimensional T1-weighted spoiled gradient-echo with and without fat suppression after administration of Gd-EOB-DTPA, and diffusion-weighted echo planar images. Rabbits were killed, and the tumor was harvested and sliced at 4-mm intervals in the axial plane. The MRI parameters evaluated were tumor size, tumor delineation, and tumor apparent diffusion coefficient (ADC) values. Histologic sections were evaluated to quantify tumor necrosis. Results:: On contrast-enhanced MRI (obtained from 11 rabbits), the mean tumor sizes were 20, 19, and 20 mm in the arterial, portal venous, and delayed phases, respectively. Tumor delineation was most distinguishable in the delayed phase. On diffusion-weighted MRI (acquired in 13 rabbits), the mean tumor ADC value was 1.84 × 10 mm/s. The mean tumor size at pathology was 16 mm. The mean percent necrosis at the tumor's pathologic condition was 36%. The correlation between ADC value and percent necrosis showed an R value of 0.68. Conclusions:: Contrast-enhanced MRI using Gd-EOB-DTPA may provide additional information about tumor outline in the liver. Moreover, we showed a remarkable correlation between ADC values and tumor necrosis. Thus, diffusion-weighted imaging may be useful to assess tumor necrosis; nevertheless, the search for new modalities remains important.

KW - Diffusion-weighted MRI

KW - Gd-EOB-DTPA

KW - HCC

KW - Liver cancer

KW - Tumor necrosis

KW - Tumor size

KW - VX2

UR - http://www.scopus.com/inward/record.url?scp=68949144862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949144862&partnerID=8YFLogxK

U2 - 10.1097/RCT.0b013e3181953df3

DO - 10.1097/RCT.0b013e3181953df3

M3 - Article

VL - 33

SP - 626

EP - 630

JO - Journal of Computer Assisted Tomography

JF - Journal of Computer Assisted Tomography

SN - 0363-8715

IS - 4

ER -