Diffusion tensor imaging of diffuse axonal injury in a rat brain trauma model

Yohan van de Looij, Franck Mauconduit, Marine Beaumont, Samuel Valable, Régine Farion, Gilles Francony, Jean François Payen, Hana Lahrech

Research output: Contribution to journalArticle

Abstract

Diffusion tensor imaging (DTI) was used to study traumatic brain injury. The impact-acceleration trauma model was used in rats. Here, in addition to diffusivities (mean, axial and radial), fractional anisotropy (FA) was used, in particular, as a parameter to characterize the cerebral tissue early after trauma. DTI was implemented at 7T using fast spiral k-space sampling and the twice-refocused spin echo radiofrequency sequence for eddy current minimization. The method was carefully validated on different phantom measurements. DTI of a trauma group (n=5), as well as a sham group (n=5), was performed at different time points during 6h following traumatic brain injury. Two cerebral regions, the cortex and corpus callosum, were analyzed carefully. A significant decrease in diffusivity in the trauma group versus the sham group was observed, suggesting the predominance of cellular edema in both cerebral regions. No significant FA change was detected in the cortex. In the corpus callosum of the trauma group, the FA indices were significantly lower. A net discontinuity in fiber reconstructions in the corpus callosum was observed by fiber tracking using DTI. Histological analysis using Hoechst, myelin basic protein and Bielschowsky staining showed fiber disorganization in the corpus callosum in the brains of the trauma group. On the basis of our histology results and the characteristics of the impact-acceleration model responsible for the presence of diffuse axonal injury, the detection of low FA caused by a drastic reduction in axial diffusivity and the presence of fiber disconnections of the DTI track in the corpus callosum were considered to be related to the presence of diffuse axonal injury.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalNMR in biomedicine
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • Diffuse axonal injury
  • Diffusion tensor imaging
  • Fiber tracking
  • Fractional anisotropy
  • Impact-acceleration model
  • Rat brain
  • Traumatic brain injury

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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  • Cite this

    van de Looij, Y., Mauconduit, F., Beaumont, M., Valable, S., Farion, R., Francony, G., Payen, J. F., & Lahrech, H. (2012). Diffusion tensor imaging of diffuse axonal injury in a rat brain trauma model. NMR in biomedicine, 25(1), 93-103. https://doi.org/10.1002/nbm.1721