Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains

Eric H. Chang, Miklos Argyelan, Manisha Aggarwal, Toni Shay S. Chandon, Katherine H. Karlsgodt, Susumu Mori, Anil K. Malhotra

Research output: Contribution to journalArticlepeer-review

Abstract

We provide datasets from combined ex vivo diffusion tensor imaging (DTI) and Clear Lipid-exchanged, Anatomically Rigid, Imaging/immunostaining compatible, Tissue hYdrogel (CLARITY) performed on intact mouse brains. DTI-derived measures of fractional anisotropy (FA), radial diffusivity (RD), and axial diffusivity (AD) were compared to antibody-based labeling of myelin basic protein (MBP), as measured by fluorescence microscopy. We used a customized CLARITY hydrogel solution to facilitate whole brain tissue clearing and subsequent immunolabeling. We describe how CLARITY was made compatible with magnetic resonance imaging with the intention of facilitating future multimodal imaging studies that may combine noninvasive imaging with 3D immunohistochemistry. These data and methods are related to the accompanying research article entitled, ‘The role of myelination in measures of white matter integrity: Combination of diffusion tensor imaging and two-photon microscopy of CLARITY intact brains’ (E.H. Chang, M. Argyelan, M. Aggarwal, T-S. Chandon, K.H. Karlsgodt, S. Mori, A.K. Malhotra, 2016) [1].

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalData in Brief
Volume10
DOIs
StatePublished - Feb 1 2017

Keywords

  • CLARITY
  • DTI
  • Multimodal imaging
  • Myelination
  • Radial diffusivity

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains'. Together they form a unique fingerprint.

Cite this