Diffusion rates of cell surface antigens of mouse- human heterokaryons: I. analysis of the population

Michael Edidin, Taiyin Wei

Research output: Contribution to journalArticlepeer-review

Abstract

The rate of appearance, in a newly formed heterokaryon population, of cells bearing completely intermixed mouse and human surface antigens may be used to estimate diffusion constants for antigens on individual cells. From this estimate, it appears that the surface antigens in most cells do not diffuse at the rate expected, but rather move more slowly, by a factor of ten or more, than expected from either measured or calculated diffusion constants for proteins freely mobile in the plane of a lipid membrane. Differences in diffusion rates between cells are not due to effects of Sendai virus, or of trypsin. Restrictions on diffusion are apparently not due to cytochalasin B- or Colcemid-sensitive elements.

Original languageEnglish (US)
Pages (from-to)75-482
Number of pages408
JournalJournal of Cell Biology
Volume75
Issue number2
DOIs
StatePublished - Nov 1 1977

Keywords

  • Cell surface heterokaryons ·
  • Diffusion ·
  • Sendai virus
  • Surface antigens ·

ASJC Scopus subject areas

  • Cell Biology

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