TY - JOUR
T1 - Diffusion rates of cell surface antigens of mouse- human heterokaryons
T2 - II. effect of membrane potential on lateral diffusion
AU - Edidin, Michael
AU - Wei, Taiyin Wei
PY - 1977/11/1
Y1 - 1977/11/1
N2 - The rate of appearance, in a population of mouse-human heterokaryons, of cells with intermixed mouse and human surface antigens may be used to estimate the rate of lateral diffusion of the antigens in a single cell. Most heterokaryons appear to restrict diffusion of their surface antigens. These restrictions are altered by exposing either heterokaryons or their parent cells to conditions that change cell surface membrane potential. Media containing unphysiological concentrations of potassium ion, drugs, affecting the Na+, K+ ATPase, or a channel-forming antibiotic, gramicidin, all affect lateral mobility of cell surface antigens in a manner consistent with a common effect on membrane potential.
AB - The rate of appearance, in a population of mouse-human heterokaryons, of cells with intermixed mouse and human surface antigens may be used to estimate the rate of lateral diffusion of the antigens in a single cell. Most heterokaryons appear to restrict diffusion of their surface antigens. These restrictions are altered by exposing either heterokaryons or their parent cells to conditions that change cell surface membrane potential. Media containing unphysiological concentrations of potassium ion, drugs, affecting the Na+, K+ ATPase, or a channel-forming antibiotic, gramicidin, all affect lateral mobility of cell surface antigens in a manner consistent with a common effect on membrane potential.
KW - Cell surface ·
KW - Diffusion ·
KW - Heterokaryons ·
KW - Membrane potential ·
KW - Surface antigens
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U2 - 10.1083/jcb.75.2.483
DO - 10.1083/jcb.75.2.483
M3 - Article
C2 - 95669
AN - SCOPUS:0017648977
SN - 0021-9525
VL - 75
SP - 483
EP - 489
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -