Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retinoic acid

L. A. Doyle, D. Giangiulo, A. Hussain, H. J. Park, Ray-Whay Yen, M. Borges

Research output: Contribution to journalArticle

Abstract

Variant small cell lung cancer (SCLC) is distinguished from the classic histology by changes in growth characteristics and morphology, c-myc amplification, a loss of some biochemical markers, and relative chemo- and radioresistance. Three variant SCLC lines were incubated in 1 μM all-trans retinoic acid. After 8-10 days, a marked change in morphology was noted in all three lines, with tight cell aggregation and central necrosis of large floating spheroids similar to classic SCLC. The retinoid-treated cells demonstrated decreases in growth rate and cloning efficiency to levels comparable with classic SCLC cell lines. Retinoic acid incubation caused a reproducible decrease in c-myc expression in variant SCLC cells, but was not noted to increase L-dopa carboxylase, an enzyme which biochemically distinguishes classic from variant SCLC cell lines. Retinoid exposure led to an increase in HLA and Leu-7 antigens, but a reduction of antibody binding to 3-fucosyllactosamine, a dominant SCLC glycolipid antigen. Clonogenic assays revealed that the variant cells, after incubation in retinoic acid, became more sensitive to etoposide, but more resistant to Adriamycin. We conclude that exposure of variant SCLC cells to retinoic acid can lead to a morphologic phenotype similar to classic SCLC, but with substantial differences in cell biology.

Original languageEnglish (US)
Pages (from-to)6745-6751
Number of pages7
JournalCancer Research
Volume49
Issue number23
StatePublished - 1989
Externally publishedYes

Fingerprint

Small Cell Lung Carcinoma
Tretinoin
Cell Line
Retinoids
CD57 Antigens
Cell Aggregation
Glycolipids
Levodopa
Etoposide
Growth
Doxorubicin
Cell Biology
Organism Cloning
Histology
Necrosis
Biomarkers
Phenotype
Antigens
Antibodies
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Doyle, L. A., Giangiulo, D., Hussain, A., Park, H. J., Yen, R-W., & Borges, M. (1989). Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retinoic acid. Cancer Research, 49(23), 6745-6751.

Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retinoic acid. / Doyle, L. A.; Giangiulo, D.; Hussain, A.; Park, H. J.; Yen, Ray-Whay; Borges, M.

In: Cancer Research, Vol. 49, No. 23, 1989, p. 6745-6751.

Research output: Contribution to journalArticle

Doyle, LA, Giangiulo, D, Hussain, A, Park, HJ, Yen, R-W & Borges, M 1989, 'Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retinoic acid', Cancer Research, vol. 49, no. 23, pp. 6745-6751.
Doyle, L. A. ; Giangiulo, D. ; Hussain, A. ; Park, H. J. ; Yen, Ray-Whay ; Borges, M. / Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retinoic acid. In: Cancer Research. 1989 ; Vol. 49, No. 23. pp. 6745-6751.
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