Differentiating Streptococcus pseudoporcinus from GBS: could this have implications in pregnancy?

Maureen Grundy, Nuntra Suwantarat, Mayer Rubin, Renee Harris, Ann Hanlon, Tsigereda Tekle, Brandon Ellis, Karen C Carroll, Frank R Witter

Research output: Contribution to journalArticle

Abstract

Background: Streptococcus agalactiae (GBS) is a common pathogen known to cause neonatal and maternal infectious morbidity. Streptococcus pseudoporcinus (S pseudoporcinus) is a separate, recently identified β-hemolytic gram-positive coccus that can cause false-positive results on standard GBS agglutination testing assays. Objective: To determine the prevalence and clinical implications of Streptococcus pseudoporcinus colonization in pregnancy. Materials and Methods: This is a 2-year retrospective cohort study comparing pregnant women colonized with GBS to those colonized with S. pseudoporcinus. A proteomics method of identification, namely, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, was used to distinguish between S. pseudoporcinus and GBS colonization. Antibiotic susceptibility testing was carried out on all specimens. Maternal and neonatal chart reviews were conducted to identify predictors of S. pseudoporcinus colonization and to compare maternal and neonatal outcomes. Results: S. pseudoporcinus colonization occurred in 1.6% of all pregnancies. A total of 2.5% of all GBS-positive results by agglutination assay were false positive, instead reflecting S. pseudoporcinus colonization. Clindamycin resistance among S. pseudoporcinus isolates is uncommon. S. pseudoporcinus colonization in pregnancy is independently associated with African American race, tobacco use, and body mass index ≥35. Preterm premature rupture of membranes or spontaneous preterm birth was more common in patients colonized with S. pseudoporcinus. Conclusion: Although the prevalence of S. pseudoporcinus colonization is low, it primarily occurs in African American women and is associated with preterm premature rupture of membranes or spontaneous preterm birth when compared to individuals colonized with GBS.

Original languageEnglish (US)
JournalAmerican journal of obstetrics and gynecology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Streptococcus
Agglutination
Premature Birth
Mothers
African Americans
Pregnancy
Gram-Positive Cocci
Streptococcus agalactiae
Clindamycin
Tobacco Use
Proteomics
Pregnant Women
Mass Spectrometry
Lasers
Body Mass Index
Cohort Studies
Retrospective Studies
Anti-Bacterial Agents
Morbidity
Preterm Premature Rupture of the Membranes

Keywords

  • GBS
  • GBS colonization in pregnancy
  • preterm birth
  • preterm premature rupture of membranes
  • Streptococcus agalactiae
  • Streptococcus pseudoporcinus

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Differentiating Streptococcus pseudoporcinus from GBS : could this have implications in pregnancy? / Grundy, Maureen; Suwantarat, Nuntra; Rubin, Mayer; Harris, Renee; Hanlon, Ann; Tekle, Tsigereda; Ellis, Brandon; Carroll, Karen C; Witter, Frank R.

In: American journal of obstetrics and gynecology, 01.01.2019.

Research output: Contribution to journalArticle

Grundy, Maureen ; Suwantarat, Nuntra ; Rubin, Mayer ; Harris, Renee ; Hanlon, Ann ; Tekle, Tsigereda ; Ellis, Brandon ; Carroll, Karen C ; Witter, Frank R. / Differentiating Streptococcus pseudoporcinus from GBS : could this have implications in pregnancy?. In: American journal of obstetrics and gynecology. 2019.
@article{0157be1e585e4238bcad670e9b7aecd1,
title = "Differentiating Streptococcus pseudoporcinus from GBS: could this have implications in pregnancy?",
abstract = "Background: Streptococcus agalactiae (GBS) is a common pathogen known to cause neonatal and maternal infectious morbidity. Streptococcus pseudoporcinus (S pseudoporcinus) is a separate, recently identified β-hemolytic gram-positive coccus that can cause false-positive results on standard GBS agglutination testing assays. Objective: To determine the prevalence and clinical implications of Streptococcus pseudoporcinus colonization in pregnancy. Materials and Methods: This is a 2-year retrospective cohort study comparing pregnant women colonized with GBS to those colonized with S. pseudoporcinus. A proteomics method of identification, namely, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, was used to distinguish between S. pseudoporcinus and GBS colonization. Antibiotic susceptibility testing was carried out on all specimens. Maternal and neonatal chart reviews were conducted to identify predictors of S. pseudoporcinus colonization and to compare maternal and neonatal outcomes. Results: S. pseudoporcinus colonization occurred in 1.6{\%} of all pregnancies. A total of 2.5{\%} of all GBS-positive results by agglutination assay were false positive, instead reflecting S. pseudoporcinus colonization. Clindamycin resistance among S. pseudoporcinus isolates is uncommon. S. pseudoporcinus colonization in pregnancy is independently associated with African American race, tobacco use, and body mass index ≥35. Preterm premature rupture of membranes or spontaneous preterm birth was more common in patients colonized with S. pseudoporcinus. Conclusion: Although the prevalence of S. pseudoporcinus colonization is low, it primarily occurs in African American women and is associated with preterm premature rupture of membranes or spontaneous preterm birth when compared to individuals colonized with GBS.",
keywords = "GBS, GBS colonization in pregnancy, preterm birth, preterm premature rupture of membranes, Streptococcus agalactiae, Streptococcus pseudoporcinus",
author = "Maureen Grundy and Nuntra Suwantarat and Mayer Rubin and Renee Harris and Ann Hanlon and Tsigereda Tekle and Brandon Ellis and Carroll, {Karen C} and Witter, {Frank R}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.ajog.2019.01.219",
language = "English (US)",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",

}

TY - JOUR

T1 - Differentiating Streptococcus pseudoporcinus from GBS

T2 - could this have implications in pregnancy?

AU - Grundy, Maureen

AU - Suwantarat, Nuntra

AU - Rubin, Mayer

AU - Harris, Renee

AU - Hanlon, Ann

AU - Tekle, Tsigereda

AU - Ellis, Brandon

AU - Carroll, Karen C

AU - Witter, Frank R

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Streptococcus agalactiae (GBS) is a common pathogen known to cause neonatal and maternal infectious morbidity. Streptococcus pseudoporcinus (S pseudoporcinus) is a separate, recently identified β-hemolytic gram-positive coccus that can cause false-positive results on standard GBS agglutination testing assays. Objective: To determine the prevalence and clinical implications of Streptococcus pseudoporcinus colonization in pregnancy. Materials and Methods: This is a 2-year retrospective cohort study comparing pregnant women colonized with GBS to those colonized with S. pseudoporcinus. A proteomics method of identification, namely, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, was used to distinguish between S. pseudoporcinus and GBS colonization. Antibiotic susceptibility testing was carried out on all specimens. Maternal and neonatal chart reviews were conducted to identify predictors of S. pseudoporcinus colonization and to compare maternal and neonatal outcomes. Results: S. pseudoporcinus colonization occurred in 1.6% of all pregnancies. A total of 2.5% of all GBS-positive results by agglutination assay were false positive, instead reflecting S. pseudoporcinus colonization. Clindamycin resistance among S. pseudoporcinus isolates is uncommon. S. pseudoporcinus colonization in pregnancy is independently associated with African American race, tobacco use, and body mass index ≥35. Preterm premature rupture of membranes or spontaneous preterm birth was more common in patients colonized with S. pseudoporcinus. Conclusion: Although the prevalence of S. pseudoporcinus colonization is low, it primarily occurs in African American women and is associated with preterm premature rupture of membranes or spontaneous preterm birth when compared to individuals colonized with GBS.

AB - Background: Streptococcus agalactiae (GBS) is a common pathogen known to cause neonatal and maternal infectious morbidity. Streptococcus pseudoporcinus (S pseudoporcinus) is a separate, recently identified β-hemolytic gram-positive coccus that can cause false-positive results on standard GBS agglutination testing assays. Objective: To determine the prevalence and clinical implications of Streptococcus pseudoporcinus colonization in pregnancy. Materials and Methods: This is a 2-year retrospective cohort study comparing pregnant women colonized with GBS to those colonized with S. pseudoporcinus. A proteomics method of identification, namely, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, was used to distinguish between S. pseudoporcinus and GBS colonization. Antibiotic susceptibility testing was carried out on all specimens. Maternal and neonatal chart reviews were conducted to identify predictors of S. pseudoporcinus colonization and to compare maternal and neonatal outcomes. Results: S. pseudoporcinus colonization occurred in 1.6% of all pregnancies. A total of 2.5% of all GBS-positive results by agglutination assay were false positive, instead reflecting S. pseudoporcinus colonization. Clindamycin resistance among S. pseudoporcinus isolates is uncommon. S. pseudoporcinus colonization in pregnancy is independently associated with African American race, tobacco use, and body mass index ≥35. Preterm premature rupture of membranes or spontaneous preterm birth was more common in patients colonized with S. pseudoporcinus. Conclusion: Although the prevalence of S. pseudoporcinus colonization is low, it primarily occurs in African American women and is associated with preterm premature rupture of membranes or spontaneous preterm birth when compared to individuals colonized with GBS.

KW - GBS

KW - GBS colonization in pregnancy

KW - preterm birth

KW - preterm premature rupture of membranes

KW - Streptococcus agalactiae

KW - Streptococcus pseudoporcinus

UR - http://www.scopus.com/inward/record.url?scp=85062808798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062808798&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2019.01.219

DO - 10.1016/j.ajog.2019.01.219

M3 - Article

C2 - 30690012

AN - SCOPUS:85062808798

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

ER -