Differentiating nicotine- versus schizophrenia-associated decreases of the α7 nicotinic acetylcholine receptor transcript, CHRFAM7A, in peripheral blood lymphocytes

Emily G. Severance, Faith B. Dickerson, Cassie R. Stallings, Andrea E. Origoni, Anne Sullens, Eric T. Monson, Robert H. Yolken

Research output: Contribution to journalArticlepeer-review

Abstract

Nicotine addiction is prevalent in individuals with schizophrenia. Nicotine activation of nicotinic receptors (nAChRs) is time- and dose-dependent, but gene expression analyses often rely on qualitative self- or family-reported measures of smoking. We sought lymphocyte surrogates for cerebral α7-nAChR activity and tested if receptor transcription correlated with concurrently measured serum biomarkers for smoking [cotinine, C-reactive protein (CRP)]. PCR surveys to detect lymphocytic α7-related isoforms identified CHRFAM7A as the only consistently amplifiable transcript. In 20 smoking-matched people (n = 10 schizophrenia, n = 10 controls), we found significantly lower CHRFAM7A in cotinine and self-reported smokers versus nonsmokers (p ≤ 0.001-0.03) and an inverse correlation of cotinine with CHRFAM7A (p ≤ 0.04) in regression models. CHRFAM7A was not associated with diagnosis or CRP in any bi- or multi-variate analysis. Smoking-related CRP elevations only occurred in cotinine-based comparisons (p ≤ 0.03), and not when smoking was self-reported. Including biochemical indicators of serum nicotine can help differentiate smoking- versus disease-associated changes in nAChR expression.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalJournal of Neural Transmission
Volume116
Issue number2
DOIs
StatePublished - Feb 1 2009

Keywords

  • Alpha7 nicotinic receptor
  • Biomarker
  • Inflammation
  • Nicotinic acetylcholine receptor
  • Peripheral blood monocytes
  • Smoking

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

Fingerprint Dive into the research topics of 'Differentiating nicotine- versus schizophrenia-associated decreases of the α7 nicotinic acetylcholine receptor transcript, CHRFAM7A, in peripheral blood lymphocytes'. Together they form a unique fingerprint.

Cite this