Differential transcriptional profiles induced by amphotericin B formulations on human monocytes during response to hyphae of Aspergillus fumigatus

Maria Simitsopoulou, Emmanuel Roilides, Elpiniki Georgiadou, Fotini Paliogianni, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

Amphotericin B formulations possess diverse immunomodulatory properties that may contribute to the activity of phagocytes against invasive aspergillosis. In this work we provide a novel set of data on different gene transcriptional profiles of monocytes exposed to the combination of Aspergillus fumigatus and amphotericin B formulations. We used pathway-specific microarray analysis, RT-PCR analysis and enzyme-linked immunosorbent assays to compare the effects of amphotericin B deoxycholate (DAMB) at 1 μg/ml and amphotericin B lipid complex (ABLC) at 5 μg/ml to assess gene expression of immune molecules of THP-1 cells exposed to A. fumigatus hyphae (AF) for 4 h. A. fumigatus hyphae at effector/target ratio 10/1 induced mostly chemotactic factors for monocyte recruitment. DAMB at 1 μg/ml in the presence or absence of AF induced the most pronounced changes in pro-inflammatory and chemokine gene expression, while ABLC under the same conditions caused less dramatic effect. There was a reciprocal response of increased expression of the genes encoding IL-1β and IL-20 and decreased expression of IL-10, IL-2 and IL-3 in response of monocytes to both the hyphae and antifungal agents. These results demonstrate that amphotericin B formulations exert differential effects on genes encoding pro-inflammatory molecules, immunoregulatory molecules and chemokines by human monocytes during response to A. fumigatus and that these molecules may affect antifungal activity.

Original languageEnglish (US)
Pages (from-to)176-185
Number of pages10
JournalMedical Mycology
Volume49
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Aspergillus fumigatus
Hyphae
amphotericin B
Amphotericin B
monocytes
hyphae
Monocytes
Chemokines
Gene Expression
chemokines
gene expression
deoxycholic acid
Aspergillosis
interleukin-3
Antifungal Agents
Interleukin-3
Chemotactic Factors
Microarray Analysis
Phagocytes
antifungal agents

Keywords

  • chemokines
  • cytokines
  • ELISA
  • gene arrays
  • immunoregulation
  • Monocytes

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases

Cite this

Differential transcriptional profiles induced by amphotericin B formulations on human monocytes during response to hyphae of Aspergillus fumigatus. / Simitsopoulou, Maria; Roilides, Emmanuel; Georgiadou, Elpiniki; Paliogianni, Fotini; Walsh, Thomas J.

In: Medical Mycology, Vol. 49, No. 2, 02.2011, p. 176-185.

Research output: Contribution to journalArticle

Simitsopoulou, Maria ; Roilides, Emmanuel ; Georgiadou, Elpiniki ; Paliogianni, Fotini ; Walsh, Thomas J. / Differential transcriptional profiles induced by amphotericin B formulations on human monocytes during response to hyphae of Aspergillus fumigatus. In: Medical Mycology. 2011 ; Vol. 49, No. 2. pp. 176-185.
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