Differential trafficking of carboxyl isoforms of Ca2+-gated (Slo1) potassium channels

Donghui Ma, Takahiro Nakata, Guangping Zhang, Toshinori Hoshi, Min Li, Sojin Shikano

Research output: Contribution to journalArticle

Abstract

The pore-forming subunit of the large-conductance Ca2+-dependent K+ (Slo1) channel is encoded by one gene. However, the functional properties of Slo1 channels are diverse in part because of their numerous regulatory mechanisms including posttranslational modification and alternative splicing. In particular, multiple splice variants of the pore-forming subunit have been reported but their significance is only beginning to be elucidated. Here we examined the cell biological properties of the three common C-terminal isoforms that differ in the last 8 (Slo1_ERL and Slo1_VYR) or 61 residues (Slo1_DEC). We found that Slo1_DEC, the longest isoform, shows dramatically reduced surface expression compared to that of Slo1_ERL or Slo1_VYR. Immunocytochemistry revealed that a large fraction of Slo1_DEC remains localized in endoplasmic reticulum (ER). Using a GST fusion protein containing the Slo1_DEC-specific sequence, affinity purification was carried out to isolate interacting proteins. The identified proteins include protein phosphatase 2A (PP2A-A), actin, and tubulin. The PP2A-A interaction is specific to Slo1_DEC and causes a significant reduction of phosphorylation in Slo1_DEC but not Slo1_ERL or Slo1_VYR. The results together support the notion that Slo1_DEC nucleates isoform-specific protein complexes and possesses a cis element(s) for regulating trafficking of the Slo1 channels.

Original languageEnglish (US)
Pages (from-to)1000-1008
Number of pages9
JournalFEBS Letters
Volume581
Issue number5
DOIs
StatePublished - Mar 6 2007

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Keywords

  • BK
  • Potassium channels
  • Proteomics
  • Trafficking

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ma, D., Nakata, T., Zhang, G., Hoshi, T., Li, M., & Shikano, S. (2007). Differential trafficking of carboxyl isoforms of Ca2+-gated (Slo1) potassium channels. FEBS Letters, 581(5), 1000-1008. https://doi.org/10.1016/j.febslet.2007.01.077