TY - JOUR
T1 - Differential synaptic localization of GluR2 and EAAC1 in the macaque monkey entorhinal cortex
T2 - A postembedding immunogold study
AU - He, Yong
AU - Hof, Patrick R.
AU - Janssen, William G.M.
AU - Rothstein, Jeffery D.
AU - Morrison, John H.
N1 - Funding Information:
We thank R.A. Shah for help with immuno EM quantification. This work was supported by NIH grants AG05138 and AG06647, and the Charles A. Dana Foundation.
PY - 2001/10/5
Y1 - 2001/10/5
N2 - The synaptic distribution of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit GluR2 and neuronal glutamate transporter subunit EAAC1 were studied using immunogold in layer II of the macaque monkey entorhinal cortex. Immunoreactivity for EAAC1 and GluR2 was frequent at asymmetric synapses and their associated membrane. The synaptic localization of EAAC1 differed considerably from that of GluR2, in that GluR2 immunolabelling was most commonly located within the postsynaptic density, but EAAC1 localization was more heterogeneous and was predominant at the edge of postsynaptic densities and perisynaptic zones. Since EAAC1 may play an important role in clearing glutamate from the synaptic cleft and intercellular spaces, the high perisynaptic expression of EAAC1 in these neurons could presumably offer a powerful mechanism through which high concentrations of glutamate could be efficiently removed from the synapses following release and interaction with glutamate receptors. The distribution of EAAC1 may also offer protection for these neurons against excessive glutamatergic stimuli that may occur under certain pathological conditions.
AB - The synaptic distribution of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit GluR2 and neuronal glutamate transporter subunit EAAC1 were studied using immunogold in layer II of the macaque monkey entorhinal cortex. Immunoreactivity for EAAC1 and GluR2 was frequent at asymmetric synapses and their associated membrane. The synaptic localization of EAAC1 differed considerably from that of GluR2, in that GluR2 immunolabelling was most commonly located within the postsynaptic density, but EAAC1 localization was more heterogeneous and was predominant at the edge of postsynaptic densities and perisynaptic zones. Since EAAC1 may play an important role in clearing glutamate from the synaptic cleft and intercellular spaces, the high perisynaptic expression of EAAC1 in these neurons could presumably offer a powerful mechanism through which high concentrations of glutamate could be efficiently removed from the synapses following release and interaction with glutamate receptors. The distribution of EAAC1 may also offer protection for these neurons against excessive glutamatergic stimuli that may occur under certain pathological conditions.
KW - Excitatory amino acids
KW - Excitotoxicity
KW - Synaptic transmission
KW - Transporter
KW - Ultrastructure
KW - α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor
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U2 - 10.1016/S0304-3940(01)02180-2
DO - 10.1016/S0304-3940(01)02180-2
M3 - Article
C2 - 11578819
AN - SCOPUS:0035812867
SN - 0304-3940
VL - 311
SP - 161
EP - 164
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -