Differential susceptibility to experimental glaucoma among 3 mouse strains using bead and viscoelastic injection

Frances E. Cone, Scott E. Gelman, Janice L. Son, Mary Ellen Pease, Harry A Quigley

Research output: Contribution to journalArticle

Abstract

The purpose of this experiment was to test the susceptibility to retinal ganglion cell (RGC) axon loss and RGC layer cell loss from experimental glaucoma among 3 mouse strains, and between younger and older mice. We obstructed the mouse aqueous outflow channels by injecting 2 μL of 6 μm diameter, polystyrene beads followed by 3 μL of viscoelastic solution into the anterior chamber with a glass micropipette. We evaluated intraocular pressure (IOP) and damage to RGC as measured by optic nerve axon counts and RGC layer neuron counts in 3 strains of young mice (2 month old C57BL/6, DBA/2J, and CD1) and 10 month C57BL/6 mice. Bead and viscoelastic injection produced IOP elevation at ≥1 time point in 94.1% of eyes (112/119), with mean IOP difference from fellow eyes of 4.4 ± 3.0 mmHg. By 6-12 weeks, injected eyes were 10.8% longer and 7.6% wider (p <0.0001). Young DBA/2J and C57BL/6 eyes increased axial length significantly more than young CD1 or older C57BL/6 (all p ≤ 0.02). RGC layer and axon loss was greatest in CD1 mice, significantly more than the other groups (p from 0.04 to

Original languageEnglish (US)
Pages (from-to)415-424
Number of pages10
JournalExperimental Eye Research
Volume91
Issue number3
DOIs
StatePublished - Sep 2010

Fingerprint

Retinal Ganglion Cells
Glaucoma
Injections
Intraocular Pressure
Axons
Viscoelastic Substances
Eye Axial Length
Polystyrenes
Anterior Chamber
Optic Nerve
Inbred C57BL Mouse
Glass
Neurons

Keywords

  • Aqueous outflow channels
  • Experimental
  • Ganglion cells
  • Glaucoma
  • Mouse
  • Polystyrene beads
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Differential susceptibility to experimental glaucoma among 3 mouse strains using bead and viscoelastic injection. / Cone, Frances E.; Gelman, Scott E.; Son, Janice L.; Pease, Mary Ellen; Quigley, Harry A.

In: Experimental Eye Research, Vol. 91, No. 3, 09.2010, p. 415-424.

Research output: Contribution to journalArticle

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