Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells

W. C. Brown, C. Sugimoto, P. A. Conrad, D. J. Grab

Research output: Contribution to journalArticle

Abstract

T-cell lines generated from Theileria parva-immune cattle were used to identify antigens associated with schizont-infected lymphoblastoid cells. Homogenates prepared from T. parva-infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane-bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont-specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti-theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug-treated cells, demonstrating that stimulation of Theileria-specific helper and cytotoxic T-cell responses is associated with the presence of the parasite.

Original languageEnglish (US)
Pages (from-to)567-583
Number of pages17
JournalParasite Immunology
Volume11
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Theileria parva
Schizonts
T-Lymphocytes
Antigens
Cell Line
Membranes
Parasites
Theileria
Subcellular Fractions
Helper-Inducer T-Lymphocytes
Centrifugation
Mitogens
Pharmaceutical Preparations
Interleukin-2
Monoclonal Antibodies
Proteins

Keywords

  • antigens
  • cattle
  • schizont
  • T-lymphocytes
  • Theileria parva

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells. / Brown, W. C.; Sugimoto, C.; Conrad, P. A.; Grab, D. J.

In: Parasite Immunology, Vol. 11, No. 6, 1989, p. 567-583.

Research output: Contribution to journalArticle

Brown, W. C. ; Sugimoto, C. ; Conrad, P. A. ; Grab, D. J. / Differential response of bovine T-cell lines to membrane and soluble antigens of Theileria parva schizont-infected cells. In: Parasite Immunology. 1989 ; Vol. 11, No. 6. pp. 567-583.
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