T-cell lines generated from Theileria parva-immune cattle were used to identify antigens associated with schizont-infected lymphoblastoid cells. Homogenates prepared from T. parva-infected cells were fractionated by differential centrifugation, and antigenically distinct soluble and membrane-bound antigens were detected by the differential stimulation of cell lines derived from two animals. Activity in the soluble fraction was not attributable to either a mitogen or interleukin 2. Activity in the membrane fraction was associated with schizont membranes as indicated by the presence in this fraction of a parasite protein detected by immunoblot analysis using a schizont-specific monoclonal antibody. Elimination of intracellular schizonts over time, using the anti-theilerial drug, parvaquone, resulted in a concomitant loss of antigenicity in infected cells and in subcellular fractions prepared from drug-treated cells, demonstrating that stimulation of Theileria-specific helper and cytotoxic T-cell responses is associated with the presence of the parasite.
|Original language||English (US)|
|Number of pages||17|
|State||Published - 1989|
- Theileria parva
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