Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas.

John W. Lin, Andrew V. Biankin, Marko E. Horb, Bidyut Ghosh, Nijaguna B. Prasad, Nelson S. Yee, Michael A. Pack, Steven D. Leach

Research output: Contribution to journalArticle

Abstract

Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor Ptf1a-p48 in the development of both exocrine and endocrine pancreas. We have cloned the Ptf1a-p48 ortholog in Danio rerio. Early zebrafish ptf1a expression is observed in developing hindbrain and in endodermal pancreatic precursors. Analysis of ptf1a and insulin expression reveals a population of exocrine precursors that, throughout early development, are temporally and spatially segregated from endocrine elements. Morpholino-mediated knockdown of ptf1a confirms early divergence of these endocrine and exocrine lineages. Ptf1a morphants lack differentiated exocrine pancreas, but maintain normal differentiation and organization of the principal islet. In addition to the exocrine phenotype, ptf1a knockdown also reduces the prevalence of a small population of anterior endocrine cells normally found outside the principal islet. Together, these findings suggest the presence of distinct ptf1a-dependent and ptf1a-independent precursor populations in developing zebrafish pancreas.

Original languageEnglish (US)
Pages (from-to)491-503
Number of pages13
JournalDevelopmental Biology
Volume274
Issue number2
StatePublished - Oct 15 2004

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Zebrafish
Pancreas
Exocrine Pancreas
Population
Basic Helix-Loop-Helix Transcription Factors
Morpholinos
Rhombencephalon
Endocrine Cells
Islets of Langerhans
Organizations
Insulin
Phenotype

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Lin, J. W., Biankin, A. V., Horb, M. E., Ghosh, B., Prasad, N. B., Yee, N. S., ... Leach, S. D. (2004). Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. Developmental Biology, 274(2), 491-503.

Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. / Lin, John W.; Biankin, Andrew V.; Horb, Marko E.; Ghosh, Bidyut; Prasad, Nijaguna B.; Yee, Nelson S.; Pack, Michael A.; Leach, Steven D.

In: Developmental Biology, Vol. 274, No. 2, 15.10.2004, p. 491-503.

Research output: Contribution to journalArticle

Lin, JW, Biankin, AV, Horb, ME, Ghosh, B, Prasad, NB, Yee, NS, Pack, MA & Leach, SD 2004, 'Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas.', Developmental Biology, vol. 274, no. 2, pp. 491-503.
Lin JW, Biankin AV, Horb ME, Ghosh B, Prasad NB, Yee NS et al. Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. Developmental Biology. 2004 Oct 15;274(2):491-503.
Lin, John W. ; Biankin, Andrew V. ; Horb, Marko E. ; Ghosh, Bidyut ; Prasad, Nijaguna B. ; Yee, Nelson S. ; Pack, Michael A. ; Leach, Steven D. / Differential requirement for ptf1a in endocrine and exocrine lineages of developing zebrafish pancreas. In: Developmental Biology. 2004 ; Vol. 274, No. 2. pp. 491-503.
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