Differential release of prostaglandins and leukotrienes by sensitized guinea pig urinary bladder layers upon antigen challenge

R. Saban, B. J. Undem, I. M. Keith, M. R. Saban, M. W. Tengowski, F. M. Graziano, D. E. Bjorling

Research output: Contribution to journalArticle

Abstract

The relative contributions of mucosal/submucosal and detrusor layers to the release of inflammatory mediators were investigated in isolated, ovalbumin (OVA) sensitized guinea pig urinary bladders. Ovalbumin challenge of sensitized mucosa induced release of prostaglandins (PG): PGE2, PGD2 and PGF(2α), in that order of magnitude. The total release of PGs was significantly higher from the mucosa/submucosa than from the detrusor/serosa. Under the same conditions, net release of leukotriene (LT) was observed predominantly from the detrusor. The total amount of histamine released from the mucosa was greater than that from the muscle layer. These results indicate differential production and release of inflammatory mediators from the mucosal/submucosal and detrusor smooth muscle layers. These results may have serious implications in disorders, such as interstitial cystitis, involving bladder mucosal damage. The cytoprotective effect of PGE2 is likely to be lost when the mucosa is damaged, and LT release from deeper layers may contribute significantly to symptoms of bladder inflammation.

Original languageEnglish (US)
Pages (from-to)544-549
Number of pages6
JournalJournal of Urology
Volume152
Issue number2
DOIs
StatePublished - Jan 1 1994

Keywords

  • cystitis
  • eicosanoids
  • histamine
  • leukotrienes
  • prostaglandins

ASJC Scopus subject areas

  • Urology

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