Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension

Zhi Qin Wang, Lesley J. Millatt, Nicholas T. Heiderstadt, Helmy M. Siragy, Roger A Johns, Robert M. Carey

Research output: Contribution to journalArticle

Abstract

This study was designed to investigate distribution and regulation of the renal AT(1A) and AT2 subtype receptors in rats with either systemic angiotensin II (Ang II)-induced hypertension or acute phase renal hypertension (2-kidney, 1-clip [2K1C] or 2-kidney, 1-figure-of-8-wrap [2K1W]). In normal rat kidneys, positive immunostaining for the AT(1A) receptor was observed in the intrarenal vasculature, glomeruli, proximal and distal tubules, and collecting ducts. The AT2 receptor was localized mainly to the glomeruli. The AT(1A) but not AT2 receptor protein expression was significantly reduced in rats with 10-day systemic Ang II-induced hypertension. In both 7-day 2K1C and 3-day 2K1W rats, the AT(1A) receptor was significantly reduced in ischemic and contralateral kidneys compared with sham-operated control rats. Reduction in AT2 receptor expression was observed only in the ischemic kidneys in 2K1C and 2K1W renal hypertensive rats. These results demonstrate that the AT(1A) receptor is widely distributed in the glomerulus and all other nephron segments of the rat kidney. Renal AT(1A) but not AT2 receptor protein is downregulated in rats with Ang II-induced hypertension. In renal hypertensive rats, the AT(1A) receptor is bilaterally downregulated and the AT2 receptor is downregulated only in the ischemic kidney.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalHypertension
Volume33
Issue number1 I
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Angiotensins
Hypertension
Kidney
Proteins
Surgical Instruments
Angiotensin II
Down-Regulation
Renal Hypertension
Nephrons

Keywords

  • Hypertension, renal
  • Immunohistochemistry
  • Kidney
  • Receptors, angiotensin II

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Wang, Z. Q., Millatt, L. J., Heiderstadt, N. T., Siragy, H. M., Johns, R. A., & Carey, R. M. (1999). Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension. Hypertension, 33(1 I), 96-101.

Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension. / Wang, Zhi Qin; Millatt, Lesley J.; Heiderstadt, Nicholas T.; Siragy, Helmy M.; Johns, Roger A; Carey, Robert M.

In: Hypertension, Vol. 33, No. 1 I, 01.1999, p. 96-101.

Research output: Contribution to journalArticle

Wang, ZQ, Millatt, LJ, Heiderstadt, NT, Siragy, HM, Johns, RA & Carey, RM 1999, 'Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension', Hypertension, vol. 33, no. 1 I, pp. 96-101.
Wang, Zhi Qin ; Millatt, Lesley J. ; Heiderstadt, Nicholas T. ; Siragy, Helmy M. ; Johns, Roger A ; Carey, Robert M. / Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension. In: Hypertension. 1999 ; Vol. 33, No. 1 I. pp. 96-101.
@article{503174d4ea254a7aac32a6749d853242,
title = "Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension",
abstract = "This study was designed to investigate distribution and regulation of the renal AT(1A) and AT2 subtype receptors in rats with either systemic angiotensin II (Ang II)-induced hypertension or acute phase renal hypertension (2-kidney, 1-clip [2K1C] or 2-kidney, 1-figure-of-8-wrap [2K1W]). In normal rat kidneys, positive immunostaining for the AT(1A) receptor was observed in the intrarenal vasculature, glomeruli, proximal and distal tubules, and collecting ducts. The AT2 receptor was localized mainly to the glomeruli. The AT(1A) but not AT2 receptor protein expression was significantly reduced in rats with 10-day systemic Ang II-induced hypertension. In both 7-day 2K1C and 3-day 2K1W rats, the AT(1A) receptor was significantly reduced in ischemic and contralateral kidneys compared with sham-operated control rats. Reduction in AT2 receptor expression was observed only in the ischemic kidneys in 2K1C and 2K1W renal hypertensive rats. These results demonstrate that the AT(1A) receptor is widely distributed in the glomerulus and all other nephron segments of the rat kidney. Renal AT(1A) but not AT2 receptor protein is downregulated in rats with Ang II-induced hypertension. In renal hypertensive rats, the AT(1A) receptor is bilaterally downregulated and the AT2 receptor is downregulated only in the ischemic kidney.",
keywords = "Hypertension, renal, Immunohistochemistry, Kidney, Receptors, angiotensin II",
author = "Wang, {Zhi Qin} and Millatt, {Lesley J.} and Heiderstadt, {Nicholas T.} and Siragy, {Helmy M.} and Johns, {Roger A} and Carey, {Robert M.}",
year = "1999",
month = "1",
language = "English (US)",
volume = "33",
pages = "96--101",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "1 I",

}

TY - JOUR

T1 - Differential regulation of renal angiotensin subtype AT(1A) and AT2 receptor protein in rats with angiotensin-dependent hypertension

AU - Wang, Zhi Qin

AU - Millatt, Lesley J.

AU - Heiderstadt, Nicholas T.

AU - Siragy, Helmy M.

AU - Johns, Roger A

AU - Carey, Robert M.

PY - 1999/1

Y1 - 1999/1

N2 - This study was designed to investigate distribution and regulation of the renal AT(1A) and AT2 subtype receptors in rats with either systemic angiotensin II (Ang II)-induced hypertension or acute phase renal hypertension (2-kidney, 1-clip [2K1C] or 2-kidney, 1-figure-of-8-wrap [2K1W]). In normal rat kidneys, positive immunostaining for the AT(1A) receptor was observed in the intrarenal vasculature, glomeruli, proximal and distal tubules, and collecting ducts. The AT2 receptor was localized mainly to the glomeruli. The AT(1A) but not AT2 receptor protein expression was significantly reduced in rats with 10-day systemic Ang II-induced hypertension. In both 7-day 2K1C and 3-day 2K1W rats, the AT(1A) receptor was significantly reduced in ischemic and contralateral kidneys compared with sham-operated control rats. Reduction in AT2 receptor expression was observed only in the ischemic kidneys in 2K1C and 2K1W renal hypertensive rats. These results demonstrate that the AT(1A) receptor is widely distributed in the glomerulus and all other nephron segments of the rat kidney. Renal AT(1A) but not AT2 receptor protein is downregulated in rats with Ang II-induced hypertension. In renal hypertensive rats, the AT(1A) receptor is bilaterally downregulated and the AT2 receptor is downregulated only in the ischemic kidney.

AB - This study was designed to investigate distribution and regulation of the renal AT(1A) and AT2 subtype receptors in rats with either systemic angiotensin II (Ang II)-induced hypertension or acute phase renal hypertension (2-kidney, 1-clip [2K1C] or 2-kidney, 1-figure-of-8-wrap [2K1W]). In normal rat kidneys, positive immunostaining for the AT(1A) receptor was observed in the intrarenal vasculature, glomeruli, proximal and distal tubules, and collecting ducts. The AT2 receptor was localized mainly to the glomeruli. The AT(1A) but not AT2 receptor protein expression was significantly reduced in rats with 10-day systemic Ang II-induced hypertension. In both 7-day 2K1C and 3-day 2K1W rats, the AT(1A) receptor was significantly reduced in ischemic and contralateral kidneys compared with sham-operated control rats. Reduction in AT2 receptor expression was observed only in the ischemic kidneys in 2K1C and 2K1W renal hypertensive rats. These results demonstrate that the AT(1A) receptor is widely distributed in the glomerulus and all other nephron segments of the rat kidney. Renal AT(1A) but not AT2 receptor protein is downregulated in rats with Ang II-induced hypertension. In renal hypertensive rats, the AT(1A) receptor is bilaterally downregulated and the AT2 receptor is downregulated only in the ischemic kidney.

KW - Hypertension, renal

KW - Immunohistochemistry

KW - Kidney

KW - Receptors, angiotensin II

UR - http://www.scopus.com/inward/record.url?scp=0032891343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032891343&partnerID=8YFLogxK

M3 - Article

C2 - 9931088

AN - SCOPUS:0032891343

VL - 33

SP - 96

EP - 101

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 1 I

ER -