Differential nuclear matrix protein expression in prostate cancers

Correlation with pathologic stage

Yegappan Lakshmanan, Eric N P Subong, Alan Wayne Partin

Research output: Contribution to journalArticle

Abstract

Purpose: Nuclear Matrix Proteins (NMP) have been shown to be tissue and cell-type specific. Severe unique NMPs have been investigated in various cancerous tissues, including prostate bladder and kidney, and some are presently utilized as tumor markers. This study was aimed at characterizing the differential NMP expression in the pathologically more aggressive prostate cancers. Methods: High resolution two-dimensional gel electrophoresis and silver staining was used to elucidate the NMP distribution of fresh prostate cancer nuclei, obtained from 39 radical prostatectomy specimens, surgically removed from men with clinically localized prostate cancer. Based on the final pathological grading, specimens were grouped according to predicted prognosis: poor-with seminal vesicle (SV) or lymph node (LN) involvement or established capsular penetration (ECP) with gleason score >7; intermediate-organ confined (OC) or focal capsular penetration (FCP) with gleason score 7 or ECP with gleason score 6; and good- with OC or FCP and gleason score

Original languageEnglish (US)
Pages (from-to)1354-1358
Number of pages5
JournalJournal of Urology
Volume159
Issue number4
DOIs
StatePublished - Apr 1998

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Nuclear Matrix-Associated Proteins
Neoplasm Grading
Prostatic Neoplasms
Silver Staining
Seminal Vesicles
Electrophoresis, Gel, Two-Dimensional
Tumor Biomarkers
Prostatectomy
Prostate
Urinary Bladder
Lymph Nodes
Kidney

Keywords

  • Electrophoresis
  • Nuclear matrix proteins
  • Prostate cancer
  • Staging

ASJC Scopus subject areas

  • Urology

Cite this

Differential nuclear matrix protein expression in prostate cancers : Correlation with pathologic stage. / Lakshmanan, Yegappan; Subong, Eric N P; Partin, Alan Wayne.

In: Journal of Urology, Vol. 159, No. 4, 04.1998, p. 1354-1358.

Research output: Contribution to journalArticle

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abstract = "Purpose: Nuclear Matrix Proteins (NMP) have been shown to be tissue and cell-type specific. Severe unique NMPs have been investigated in various cancerous tissues, including prostate bladder and kidney, and some are presently utilized as tumor markers. This study was aimed at characterizing the differential NMP expression in the pathologically more aggressive prostate cancers. Methods: High resolution two-dimensional gel electrophoresis and silver staining was used to elucidate the NMP distribution of fresh prostate cancer nuclei, obtained from 39 radical prostatectomy specimens, surgically removed from men with clinically localized prostate cancer. Based on the final pathological grading, specimens were grouped according to predicted prognosis: poor-with seminal vesicle (SV) or lymph node (LN) involvement or established capsular penetration (ECP) with gleason score >7; intermediate-organ confined (OC) or focal capsular penetration (FCP) with gleason score 7 or ECP with gleason score 6; and good- with OC or FCP and gleason score",
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AB - Purpose: Nuclear Matrix Proteins (NMP) have been shown to be tissue and cell-type specific. Severe unique NMPs have been investigated in various cancerous tissues, including prostate bladder and kidney, and some are presently utilized as tumor markers. This study was aimed at characterizing the differential NMP expression in the pathologically more aggressive prostate cancers. Methods: High resolution two-dimensional gel electrophoresis and silver staining was used to elucidate the NMP distribution of fresh prostate cancer nuclei, obtained from 39 radical prostatectomy specimens, surgically removed from men with clinically localized prostate cancer. Based on the final pathological grading, specimens were grouped according to predicted prognosis: poor-with seminal vesicle (SV) or lymph node (LN) involvement or established capsular penetration (ECP) with gleason score >7; intermediate-organ confined (OC) or focal capsular penetration (FCP) with gleason score 7 or ECP with gleason score 6; and good- with OC or FCP and gleason score

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