Differential Mutation Production by the Decay of Incorporated Tritium Compounds in Escherichia coli

S. Person, R. C. Bockrath

Research output: Contribution to journalArticle

Abstract

We have studied the differential mutation production by the decay of incorporated tritium compounds in E. coli (WWU) using DNA-seeking precursors (H3-thymidine), RNA-seeking precursors (H3-uracil, H3-uridine), and protein-seeking precursors (H3-histidine, H3-proline). In particular we have determined the reversion frequency of an arginine locus. The reversion frequency is measured in units of revertants/surviving bacteria/H3 decay, and has an average value of 1.84 × 10-8 for H3-uridine and H3-uracil, 0.67 × 10-8 for H3-thymidine, and 0.28 × 10-8 for H3-proline and H3-histidine. Thus, the revertants are produced most effectively by H3 decays when the label is introduced in the form of an RNA precursor. The macromolecular distribution of the label shows that 5 to 8 per cent of the H3-uridine or H3-uracil is incorporated into DNA.

Original languageEnglish (US)
Pages (from-to)355-365
Number of pages11
JournalBiophysical Journal
Volume4
Issue number5
DOIs
StatePublished - 1964
Externally publishedYes

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Uracil
Tritium
Uridine
RNA Precursors
Escherichia coli
Histidine
Proline
Thymidine
Mutation
Protein Precursors
DNA
Arginine
Bacteria

ASJC Scopus subject areas

  • Biophysics

Cite this

Differential Mutation Production by the Decay of Incorporated Tritium Compounds in Escherichia coli. / Person, S.; Bockrath, R. C.

In: Biophysical Journal, Vol. 4, No. 5, 1964, p. 355-365.

Research output: Contribution to journalArticle

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