Most studies of the distribution of cell surface antigens have not resolved regional differences in antigen distribution that correspond to particular plasma membrane topographic features such as microvilli, blebs, and ruffles. Although prior labelling studies have revealed an even distribution of surface antigens, the studies described in this report indicate a different distribution pattern for individual antigens which correlates with particular surface features. B. canaliculatum hemocyanin labelled goat-anti-mouse IgG (GAMIgG) and goat-anti-rabbit IgG (GARIgG) have been used to localize the mouse major histocompatibility antigens (H-2) and a teratoma-defined antigen on the cell surface of mouse L cell fibroblasts using the SEM. Target cells labelled specifically for teratoma-defined antigens display an even distribution of hemocyanin label over their microvilli and cell body. Cells labelled for H-2 antigens display label restricted to the cell body and absent from the cell's microvilli. Indirect immunofluorescence studies using fluorescently-labelled GARIgG and GAMIgG indicate that the preferential localization of antigens is probably not due to ligand-induced redistribution of the antigens under the study. The differential distribution of the H-2 and teratoma-defined antigens may represent a distinct segregation of antigens within a plasma membrane which has been considered uniform.
|Original language||English (US)|
|Number of pages||6|
|Journal||Scanning Electron Microscopy|
|State||Published - Dec 1 1979|
ASJC Scopus subject areas
- Control and Systems Engineering