Differential intracellular sorting of immediate early gene mRNAs depends on signals in the mRNA sequence

Christopher S. Wallace, Gregory L. Lyford, Paul F Worley, Oswald Steward

Research output: Contribution to journalArticle

Abstract

This study characterizes the differential targeting of recently synthesized immediate early gene (IEG) mRNAs to neuronal cell bodies versus dendrites and tests the hypothesis that this targeting is based on signals in the encoded proteins. A single electroconvulsive seizure induces the expression of a number of IEG mRNAs in granule cells of the dentate gyrus. Most of these IEG mRNAs remain in the cell body, including two that are characterized in the present study (the mRNAs for NGFI-A and COX-2). In contrast, the mRNA for Arc moved rapidly into dendrites at an apparent rate of ~300 μm/hr. Inhibiting protein synthesis with cycloheximide did not disrupt the differential mRNA sorting, demonstrating that the differential targeting of mRNAs is not dependent on translation.

Original languageEnglish (US)
Pages (from-to)26-35
Number of pages10
JournalJournal of Neuroscience
Volume18
Issue number1
StatePublished - 1998

Fingerprint

Immediate-Early Genes
Messenger RNA
Dendrites
RNA Transport
Dentate Gyrus
Cycloheximide
Epilepsy
Proteins

Keywords

  • Arc
  • Dendrite
  • Dendritic mRNA
  • Electroconvulsive seizure
  • Immediate early gene
  • mRNA localization
  • mRNA transport

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential intracellular sorting of immediate early gene mRNAs depends on signals in the mRNA sequence. / Wallace, Christopher S.; Lyford, Gregory L.; Worley, Paul F; Steward, Oswald.

In: Journal of Neuroscience, Vol. 18, No. 1, 1998, p. 26-35.

Research output: Contribution to journalArticle

Wallace, Christopher S. ; Lyford, Gregory L. ; Worley, Paul F ; Steward, Oswald. / Differential intracellular sorting of immediate early gene mRNAs depends on signals in the mRNA sequence. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 1. pp. 26-35.
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