Differential induction of IL-12 p40 and IL-10 mRNA in human langerhans' cells and keratinocytes by in vivo occlusion, vehicle, and all-TRANS retinoic acid

Guofen Chen, Kefei Kang, Sewon Kang, Alain H. Rook, Marek Kubin, John J. Voorhees, Kevin D. Cooper

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Hydration and pharmacologic manipulation of the skin may have immunomodulatory effects. For instance, retinoic acid (RA) in vivo upregulates antigen-presenting cell (APC) activity of Langerhans' cells (LC). Objective: Our study was to determine whether RA increases LC APC activity via alteration of the potent immunoregulatory and reciprocally acting cytokines, IL-12 and IL-10. Methods: 0.1% R A and vehicle solvent only (V) as a control were applied under occlusion on the skin of normal volunteers. Freshly selected CD1a+ LC and keratinocytes from keratome were subject to semiquantitative determination of IL-12 p40 and IL-10 mRNA levels. IL-12 p40 protein was measured by radioimmunoassay. Results: Occlusion alone and open vehicle alone did not induce LC immunoregulatory cytokines; LCs demonstrated significant induction of IL-12 p40 mRNA, when the vehicle was occluded for 48 hours and, to a lesser extent, IL-10 as well. IL-12 p40 mRNA could be further induced by RA-LC at the 20-hour time point; however, IL-10 mRNA was induced at the 48-hour time point. Neither occlusion nor RA significantly induced IL-12 p40 or IL-10 mRNA in CD1a- keratinocytes at any time points. Conclusion: A tight reciprocal regulation of IL-10 and IL-12 is present in LCs and is consistent with the initial, but self-limited, inflammatory effect of occlusion and topical retinoids.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalJournal of Cutaneous Medicine and Surgery
Volume1
Issue number2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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