Differential Impact of Glucose Administered Intravenously and Orally on Circulating miR-375 Levels in Human Subjects

Xin Yan, Zhen Wang, Sidse Westberg-Rasmussen, Marcel Tarbier, Thomas Rathjen, Sudhir G. Tattikota, Bailey C.E. Peck, Matt Kanke, Claus Oxvig, Jan Frystyk, Jakob Starup-Linde, Praveen Sethupathy, Marc R. Friedländer, Søren Gregersen, Matthew Poy

Research output: Contribution to journalArticle

Abstract

Background: To date, numerous nucleic acid species have been detected in the systemic circulation including microRNAs (miRNAs); however, their functional role in this compartment remains unclear.

Objective: The aim of this study was to determine whether systemic levels of miRNAs abundant in blood, including the neuroendocrine tissue-enriched miR-375, are altered in response to a glucose challenge.

Design: Twelve healthy males were recruited for an acute crossover study that consisted of two tests each following an 8-hour fasting period. An oral glucose tolerance test (OGTT) was performed, and blood samples were collected over a 3-hour period. Following a period of at least 1 week, the same participants were administered an isoglycemic intravenous glucose infusion (IIGI) with the same blood-collection protocol.

Results: The glucose response curve following the IIGI mimicked that obtained after the OGTT, but as expected, systemic insulin levels were lower during the IIGI compared with the OGTT (P < 0.05). miR-375 levels in circulation were increased only in response to an OGTT and not during an IIGI. In addition, the response to the OGTT also coincided with the transient increase of circulating glucagon-like peptide (GLP)-1, GLP-2, and glucose-dependent insulinotropic polypeptide.

Conclusions: The present findings show levels of miR-375 increase following administration of an OGTT and, in light of its enrichment in cells of the gut, suggest that the gastrointestinal tract may play an important role in the abundance and function of this miRNA in the blood.

Original languageEnglish (US)
Pages (from-to)3749-3755
Number of pages7
JournalThe Journal of clinical endocrinology and metabolism
Volume102
Issue number10
DOIs
StatePublished - Oct 1 2017
Externally publishedYes

Fingerprint

Glucose Tolerance Test
Glucose
Intravenous Infusions
MicroRNAs
Blood
Glucagon-Like Peptide 2
Glucagon-Like Peptide 1
Cross-Over Studies
Nucleic Acids
Gastrointestinal Tract
Fasting
Insulin
Peptides
Tissue

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Differential Impact of Glucose Administered Intravenously and Orally on Circulating miR-375 Levels in Human Subjects. / Yan, Xin; Wang, Zhen; Westberg-Rasmussen, Sidse; Tarbier, Marcel; Rathjen, Thomas; Tattikota, Sudhir G.; Peck, Bailey C.E.; Kanke, Matt; Oxvig, Claus; Frystyk, Jan; Starup-Linde, Jakob; Sethupathy, Praveen; Friedländer, Marc R.; Gregersen, Søren; Poy, Matthew.

In: The Journal of clinical endocrinology and metabolism, Vol. 102, No. 10, 01.10.2017, p. 3749-3755.

Research output: Contribution to journalArticle

Yan, X, Wang, Z, Westberg-Rasmussen, S, Tarbier, M, Rathjen, T, Tattikota, SG, Peck, BCE, Kanke, M, Oxvig, C, Frystyk, J, Starup-Linde, J, Sethupathy, P, Friedländer, MR, Gregersen, S & Poy, M 2017, 'Differential Impact of Glucose Administered Intravenously and Orally on Circulating miR-375 Levels in Human Subjects', The Journal of clinical endocrinology and metabolism, vol. 102, no. 10, pp. 3749-3755. https://doi.org/10.1210/jc.2017-01365
Yan, Xin ; Wang, Zhen ; Westberg-Rasmussen, Sidse ; Tarbier, Marcel ; Rathjen, Thomas ; Tattikota, Sudhir G. ; Peck, Bailey C.E. ; Kanke, Matt ; Oxvig, Claus ; Frystyk, Jan ; Starup-Linde, Jakob ; Sethupathy, Praveen ; Friedländer, Marc R. ; Gregersen, Søren ; Poy, Matthew. / Differential Impact of Glucose Administered Intravenously and Orally on Circulating miR-375 Levels in Human Subjects. In: The Journal of clinical endocrinology and metabolism. 2017 ; Vol. 102, No. 10. pp. 3749-3755.
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AU - Yan, Xin

AU - Wang, Zhen

AU - Westberg-Rasmussen, Sidse

AU - Tarbier, Marcel

AU - Rathjen, Thomas

AU - Tattikota, Sudhir G.

AU - Peck, Bailey C.E.

AU - Kanke, Matt

AU - Oxvig, Claus

AU - Frystyk, Jan

AU - Starup-Linde, Jakob

AU - Sethupathy, Praveen

AU - Friedländer, Marc R.

AU - Gregersen, Søren

AU - Poy, Matthew

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background: To date, numerous nucleic acid species have been detected in the systemic circulation including microRNAs (miRNAs); however, their functional role in this compartment remains unclear.Objective: The aim of this study was to determine whether systemic levels of miRNAs abundant in blood, including the neuroendocrine tissue-enriched miR-375, are altered in response to a glucose challenge.Design: Twelve healthy males were recruited for an acute crossover study that consisted of two tests each following an 8-hour fasting period. An oral glucose tolerance test (OGTT) was performed, and blood samples were collected over a 3-hour period. Following a period of at least 1 week, the same participants were administered an isoglycemic intravenous glucose infusion (IIGI) with the same blood-collection protocol.Results: The glucose response curve following the IIGI mimicked that obtained after the OGTT, but as expected, systemic insulin levels were lower during the IIGI compared with the OGTT (P < 0.05). miR-375 levels in circulation were increased only in response to an OGTT and not during an IIGI. In addition, the response to the OGTT also coincided with the transient increase of circulating glucagon-like peptide (GLP)-1, GLP-2, and glucose-dependent insulinotropic polypeptide.Conclusions: The present findings show levels of miR-375 increase following administration of an OGTT and, in light of its enrichment in cells of the gut, suggest that the gastrointestinal tract may play an important role in the abundance and function of this miRNA in the blood.

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