Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain

Alan H. Sharp, Peter S. McPherson, Ted M Dawson, Chiye Aoki, Kevin P. Campbell, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Ca2+ release from inositol 1,4,5-trisphosphate(IP3)-sensitive and ryanodine-sensitive intracellular Ca2+ stores is mediated by distinct proteins identified as IP3 receptors (IP3R) and ryanodine receptors (RyR), respectively. We have compared the immunohistochemical localizations of IP3R and RyR in the brain at the light and electron microscopic levels and have also evaluated the distribution of the major brain intracellular Ca2+-pumping ATPase. IP3R and RyR occur in overlapping populations of neurons in widespread areas of the brain, but labeling is distinct in a number of areas. For example, IP3R is enriched in cerebellar Purkinje cells and hippocampal CA1 pyramidal cells, while RyR is present at relatively low levels in these cells. RyR is most enriched in the dentate gyms and CA3/4 areas of the hippocampus, where IP3R levels are low. In the cortex, IP3R is found in pyramidal cell bodies and proximal dendrites, whereas RyR is located predominantly in long, thin apical dendrites of pyramidal cells. In deep cerebellar nuclei, RyR is located in cell bodies that appear devoid of IP3R, whereas IP3R is enriched in terminals surrounding cell bodies. Electron microscopy in the hippocampus reveals RyR in axons, dendritic spines, and dendritic shafts near dendritic spines while IP3R is primarily identified in dendritic shafts and cell bodies. These results suggest that the IP3- and ryanodine-sensitive Ca2+ pools have largely distinct roles in controlling intracellular Ca2+ levels, though in some sites they may interact to varying degrees.

Original languageEnglish (US)
Pages (from-to)3051-3053
Number of pages3
JournalJournal of Neuroscience
Volume13
Issue number7
StatePublished - 1993

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Inositol 1,4,5-Trisphosphate Receptors
Ryanodine
Inositol 1,4,5-Trisphosphate
Ryanodine Receptor Calcium Release Channel
Brain
Pyramidal Cells
Dendritic Spines
Dendrites
Hippocampus
Cerebellar Nuclei
Calcium-Transporting ATPases
Purkinje Cells
Dendritic Cells
Axons
Electron Microscopy
Electrons
Neurons
Light

Keywords

  • Ca pumping ATPase
  • Ca release
  • Immuno-histochemistry
  • Inositol 1,4,5-trisphosphate receptor
  • Ryanodine receptor
  • SERCA 2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain. / Sharp, Alan H.; McPherson, Peter S.; Dawson, Ted M; Aoki, Chiye; Campbell, Kevin P.; Snyder, Solomon H.

In: Journal of Neuroscience, Vol. 13, No. 7, 1993, p. 3051-3053.

Research output: Contribution to journalArticle

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