Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models

Masayuki Iwasaki, Wen Gong Yu, Yasuhiro Uekusa, Chigusa Nakajima, Yi Fu Yang, Ping Gao, Rishani Wijesuriya, Hiromi Fujiwara, Toshiyuki Hamaoka

Research output: Contribution to journalArticle

Abstract

While IL-12 administration induces tumor regression through stimulating T cells in tumor-bearing mice, this IL-12 effect is observed in some but not all tumor models. The present study aimed to compare IL-12 responsiveness of T cells from tumor-bearing mice in IL-12-responsive (CSA1M and OV-HM) and -unresponsive (Meth A) tumor models. Tumor regression in IL-12-responsive tumor models required the participation of T cells, but not of NK1.1+ cells. Because a NK1.1+ cell population was the major producer of IFN-γ comparable levels of IFN-γ production were induced in IL-12-responsive and -unresponsive tumor-bearing mice. This indicates that the amount of IFN-γ produced in tumor-bearing individuals does not correlate with the anti-tumor efficacy of IL-12. In contrast, IL-12 responsiveness of T cells differed between the responsive and unresponsive models: purified T cells from CSA1M/OV-HM-bearing or Meth A-bearing mice exhibited high or low IL-12 responsiveness respectively, when evaluated by the amounts of IFN-γ produced in response to IL-12. T cells from CSA1M- or OV-HM-bearing but not from Meth A-bearing mice exhibited enhanced levels of mRNA for the IL-12 receptor (IL-12R). These results indicate that a fundamental difference exists in IL-12 responsiveness of T cells between IL-12-responsive and -unresponsive tumor models, and that such a difference is associated with the expression of IL-12R on T cells.

Original languageEnglish (US)
Pages (from-to)701-709
Number of pages9
JournalInternational Immunology
Volume12
Issue number5
StatePublished - 2000
Externally publishedYes

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Interleukin-12
Natural Killer Cells
T-Lymphocytes
Neoplasms
Interleukin-12 Receptors

Keywords

  • IL-12
  • NK cells
  • Tumor models

ASJC Scopus subject areas

  • Immunology

Cite this

Iwasaki, M., Yu, W. G., Uekusa, Y., Nakajima, C., Yang, Y. F., Gao, P., ... Hamaoka, T. (2000). Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models. International Immunology, 12(5), 701-709.

Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models. / Iwasaki, Masayuki; Yu, Wen Gong; Uekusa, Yasuhiro; Nakajima, Chigusa; Yang, Yi Fu; Gao, Ping; Wijesuriya, Rishani; Fujiwara, Hiromi; Hamaoka, Toshiyuki.

In: International Immunology, Vol. 12, No. 5, 2000, p. 701-709.

Research output: Contribution to journalArticle

Iwasaki, M, Yu, WG, Uekusa, Y, Nakajima, C, Yang, YF, Gao, P, Wijesuriya, R, Fujiwara, H & Hamaoka, T 2000, 'Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models', International Immunology, vol. 12, no. 5, pp. 701-709.
Iwasaki, Masayuki ; Yu, Wen Gong ; Uekusa, Yasuhiro ; Nakajima, Chigusa ; Yang, Yi Fu ; Gao, Ping ; Wijesuriya, Rishani ; Fujiwara, Hiromi ; Hamaoka, Toshiyuki. / Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models. In: International Immunology. 2000 ; Vol. 12, No. 5. pp. 701-709.
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