TY - JOUR
T1 - Differential expression of three glutamate transporter subtypes in the rat retina
AU - Rauen, T.
AU - Rothstein, J. D.
AU - Wässle, H.
PY - 1996/12/23
Y1 - 1996/12/23
N2 - The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype. A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1 ≤ EAAC-1 > GLT-1. GLAST-1 immunoreactivity was seen in Muller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.
AB - The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype. A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1 ≤ EAAC-1 > GLT-1. GLAST-1 immunoreactivity was seen in Muller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.
KW - Anti-peptide antibodies
KW - Expression
KW - Glutamate transporter subtypes (GLT-1 EAAC-1 GLAST-1)
KW - Glutamatergic pathways
KW - Immunohistochemistry
KW - Rat (Wistar, Brown Norway)
KW - Retina
UR - http://www.scopus.com/inward/record.url?scp=0029904836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029904836&partnerID=8YFLogxK
U2 - 10.1007/s004410050702
DO - 10.1007/s004410050702
M3 - Article
C2 - 8929335
AN - SCOPUS:0029904836
VL - 286
SP - 325
EP - 336
JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
SN - 0302-766X
IS - 3
ER -