Differential expression of three glutamate transporter subtypes in the rat retina

T. Rauen, J. D. Rothstein, H. Wässle

Research output: Contribution to journalArticle

Abstract

The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype. A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1 ≤ EAAC-1 > GLT-1. GLAST-1 immunoreactivity was seen in Muller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.

Original languageEnglish (US)
Pages (from-to)325-336
Number of pages12
JournalCell and Tissue Research
Volume286
Issue number3
DOIs
StatePublished - Dec 23 1996

Keywords

  • Anti-peptide antibodies
  • Expression
  • Glutamate transporter subtypes (GLT-1 EAAC-1 GLAST-1)
  • Glutamatergic pathways
  • Immunohistochemistry
  • Rat (Wistar, Brown Norway)
  • Retina

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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