Differential Expression of Interphotoreceptor Retinoid-binding Protein, Opsin, Cellular Retinaldehyde-binding Protein, and Basic Fibroblastic Growth Factor

F. Gonzalez-Fernandez, E. Van Niel, C. Edmonds, H. Beaver, J. M. Nickerson, J. M. Garcia-Fernandez, Peter A Campochiaro, R. G. Foster

Research output: Contribution to journalArticle

Abstract

The mRNA for rat interphotoreceptor retinoid-binding protein (IRBP) consists of 5·2 kb and 6·4 kb transcripts which are expressed early during retinal development. Here, we characterized rat IRBP cDNA and genomic clones, determined the mechanism generating the two transcripts and compared their expression to mRNAs for opsin, cellular retinaldehyde-binding protein (CRAIBP) and basic fibroblast growth factor (bFGF). We found that human and rat IRBPs have a high degree of sequence homology (86% amino acid identity over the fourth repeat). RNA-PCR studies and Northern blot analysis, showed that the 6·4 kb mRNA has a longer 3′-untranslated region (UTR) than the 5·2 kb message. Both IRBP transcripts, but not the mRNA for opsin are present in the neonatal and adult pineal. During development, the mRNAs for IRBP and opsin reach one-half of their maximal levels by P5 and P11, respectively. The ratio of the two IRBP mRNAs remains constant throughout life. In contrast, the ratio of the longest to the shortest opsin mRNA decreases between E19 and 1 year of age. Immuno-histochemistry demonstrated a marked increase in the amount of IRBP in the interphotoreceptor matrix between P1 and P9 corresponding to the enhanced expression of this mRNA. Up-regulation of opsin mRNA expression during the second postnatal week corresponds to the emergence of the outer segments. The temporal expression of CRAIBP is similar to IRBP while bFGF is not expressed until after photoreceptor differentiation is complete. In summary, two 3′-transcription termination sites explain the difference in IRBP mRNA sizes. The different temporal and tissue specific patterns of IRBP and opsin expression suggest that separate mechanisms control the expression of these two genes. The expression patterns of IRBP and CRAIBP are consistent with the role of vitamin A as a morphogen and bFGF in neuronal maintenance.

Original languageEnglish (US)
Pages (from-to)411-427
Number of pages17
JournalExperimental Eye Research
Volume56
Issue number4
DOIs
StatePublished - Apr 1993

Fingerprint

Retinaldehyde
Opsins
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Messenger RNA
Fibroblast Growth Factor 2
interstitial retinol-binding protein
Amino Acid Sequence Homology
3' Untranslated Regions
Vitamin A
Northern Blotting
Up-Regulation
Complementary DNA
Clone Cells
Maintenance
RNA

Keywords

  • cellular retinaldehyde-binding protein
  • fibroblastic growth factor
  • interphotoreceptor retinoid-binding protein
  • opsin
  • retinal development

ASJC Scopus subject areas

  • Sensory Systems
  • Ophthalmology

Cite this

Gonzalez-Fernandez, F., Van Niel, E., Edmonds, C., Beaver, H., Nickerson, J. M., Garcia-Fernandez, J. M., ... Foster, R. G. (1993). Differential Expression of Interphotoreceptor Retinoid-binding Protein, Opsin, Cellular Retinaldehyde-binding Protein, and Basic Fibroblastic Growth Factor. Experimental Eye Research, 56(4), 411-427. https://doi.org/10.1006/exer.1993.1055

Differential Expression of Interphotoreceptor Retinoid-binding Protein, Opsin, Cellular Retinaldehyde-binding Protein, and Basic Fibroblastic Growth Factor. / Gonzalez-Fernandez, F.; Van Niel, E.; Edmonds, C.; Beaver, H.; Nickerson, J. M.; Garcia-Fernandez, J. M.; Campochiaro, Peter A; Foster, R. G.

In: Experimental Eye Research, Vol. 56, No. 4, 04.1993, p. 411-427.

Research output: Contribution to journalArticle

Gonzalez-Fernandez, F. ; Van Niel, E. ; Edmonds, C. ; Beaver, H. ; Nickerson, J. M. ; Garcia-Fernandez, J. M. ; Campochiaro, Peter A ; Foster, R. G. / Differential Expression of Interphotoreceptor Retinoid-binding Protein, Opsin, Cellular Retinaldehyde-binding Protein, and Basic Fibroblastic Growth Factor. In: Experimental Eye Research. 1993 ; Vol. 56, No. 4. pp. 411-427.
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abstract = "The mRNA for rat interphotoreceptor retinoid-binding protein (IRBP) consists of 5·2 kb and 6·4 kb transcripts which are expressed early during retinal development. Here, we characterized rat IRBP cDNA and genomic clones, determined the mechanism generating the two transcripts and compared their expression to mRNAs for opsin, cellular retinaldehyde-binding protein (CRAIBP) and basic fibroblast growth factor (bFGF). We found that human and rat IRBPs have a high degree of sequence homology (86{\%} amino acid identity over the fourth repeat). RNA-PCR studies and Northern blot analysis, showed that the 6·4 kb mRNA has a longer 3′-untranslated region (UTR) than the 5·2 kb message. Both IRBP transcripts, but not the mRNA for opsin are present in the neonatal and adult pineal. During development, the mRNAs for IRBP and opsin reach one-half of their maximal levels by P5 and P11, respectively. The ratio of the two IRBP mRNAs remains constant throughout life. In contrast, the ratio of the longest to the shortest opsin mRNA decreases between E19 and 1 year of age. Immuno-histochemistry demonstrated a marked increase in the amount of IRBP in the interphotoreceptor matrix between P1 and P9 corresponding to the enhanced expression of this mRNA. Up-regulation of opsin mRNA expression during the second postnatal week corresponds to the emergence of the outer segments. The temporal expression of CRAIBP is similar to IRBP while bFGF is not expressed until after photoreceptor differentiation is complete. In summary, two 3′-transcription termination sites explain the difference in IRBP mRNA sizes. The different temporal and tissue specific patterns of IRBP and opsin expression suggest that separate mechanisms control the expression of these two genes. The expression patterns of IRBP and CRAIBP are consistent with the role of vitamin A as a morphogen and bFGF in neuronal maintenance.",
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