Abstract
DNA polymerases may be differentially expressed by cells during periods of quiescence and proliferation. Murine B cells are an ideal population to study because their division time varies widely in vivo, and different subsets can be easily isolated. Consequently, we analyzed RNA from resting cells (B220+ peanut agglutinin-) and activated germinal center cells (B220+ peanut agglutinin+) from spleens by reverse transcriptase-PCR using primers for five nuclear polymerases and their associated subunits. Gel analyses of the amplified products showed that the rapidly-dividing germinal center B cells expressed DNA polymerases α, β, δ, ε, and ζ. The resting B cells did not express polymerases α or ε at detectable levels, although they did express polymerases β, δ, and ζ. Thus, polymerase ε, as well as α, appears to have a primary role in chromosomal replication of murine B lymphocytes. Further, the lack of expression of polymerase ε in resting cells indicates that this enzyme is not used in any DNA repair pathways by these cells. The expression of polymerase ζ by resting cells suggests that it has another role in DNA repair, perhaps recombination, in addition to its function of bypassing damage during chromosomal replication. Published by Elsevier Science Ltd.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-131 |
| Number of pages | 7 |
| Journal | Molecular Immunology |
| Volume | 37 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - 2000 |
| Externally published | Yes |
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Keywords
- Activated
- B lymphocytes
- DNA polymerases
- Repair
- Replication
- Resting
ASJC Scopus subject areas
- Molecular Biology
- Immunology
Cite this
Differential expression of DNA polymerase ε in resting and activated B lymphocytes is consistent with an in vivo role in replication and not repair. / Winter, David B.; Phung, Quy H.; Wood, Richard D.; Gearhart, Patricia J.
In: Molecular Immunology, Vol. 37, No. 3-4, 2000, p. 125-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential expression of DNA polymerase ε in resting and activated B lymphocytes is consistent with an in vivo role in replication and not repair
AU - Winter, David B.
AU - Phung, Quy H.
AU - Wood, Richard D.
AU - Gearhart, Patricia J.
PY - 2000
Y1 - 2000
N2 - DNA polymerases may be differentially expressed by cells during periods of quiescence and proliferation. Murine B cells are an ideal population to study because their division time varies widely in vivo, and different subsets can be easily isolated. Consequently, we analyzed RNA from resting cells (B220+ peanut agglutinin-) and activated germinal center cells (B220+ peanut agglutinin+) from spleens by reverse transcriptase-PCR using primers for five nuclear polymerases and their associated subunits. Gel analyses of the amplified products showed that the rapidly-dividing germinal center B cells expressed DNA polymerases α, β, δ, ε, and ζ. The resting B cells did not express polymerases α or ε at detectable levels, although they did express polymerases β, δ, and ζ. Thus, polymerase ε, as well as α, appears to have a primary role in chromosomal replication of murine B lymphocytes. Further, the lack of expression of polymerase ε in resting cells indicates that this enzyme is not used in any DNA repair pathways by these cells. The expression of polymerase ζ by resting cells suggests that it has another role in DNA repair, perhaps recombination, in addition to its function of bypassing damage during chromosomal replication. Published by Elsevier Science Ltd.
AB - DNA polymerases may be differentially expressed by cells during periods of quiescence and proliferation. Murine B cells are an ideal population to study because their division time varies widely in vivo, and different subsets can be easily isolated. Consequently, we analyzed RNA from resting cells (B220+ peanut agglutinin-) and activated germinal center cells (B220+ peanut agglutinin+) from spleens by reverse transcriptase-PCR using primers for five nuclear polymerases and their associated subunits. Gel analyses of the amplified products showed that the rapidly-dividing germinal center B cells expressed DNA polymerases α, β, δ, ε, and ζ. The resting B cells did not express polymerases α or ε at detectable levels, although they did express polymerases β, δ, and ζ. Thus, polymerase ε, as well as α, appears to have a primary role in chromosomal replication of murine B lymphocytes. Further, the lack of expression of polymerase ε in resting cells indicates that this enzyme is not used in any DNA repair pathways by these cells. The expression of polymerase ζ by resting cells suggests that it has another role in DNA repair, perhaps recombination, in addition to its function of bypassing damage during chromosomal replication. Published by Elsevier Science Ltd.
KW - Activated
KW - B lymphocytes
KW - DNA polymerases
KW - Repair
KW - Replication
KW - Resting
UR - http://www.scopus.com/inward/record.url?scp=0034124439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034124439&partnerID=8YFLogxK
U2 - 10.1016/S0161-5890(00)00040-7
DO - 10.1016/S0161-5890(00)00040-7
M3 - Article
VL - 37
SP - 125
EP - 131
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 3-4
ER -