TY - JOUR
T1 - Differential expression of apoptosis related proteins and nitric oxide synthases in Epstein Barr associated gastric carcinomas
AU - Begnami, Maria D.
AU - Montagnini, Andre L.
AU - Vettore, Andre L.
AU - Nonogaki, Sueli
AU - Brait, Mariana
AU - Simoes-Sato, Alex Y.
AU - Seixas, Andre Q.A.
AU - Soares, Fernando A.
PY - 2006/8/21
Y1 - 2006/8/21
N2 - Aim: To determine the incidence of Epstein Barr virus associated gastric carcinoma (GC) in Brazil and compare the expressions of apoptosis related proteins and nitric oxide synthases between EBV positive and negative gastric carcinoma. Methods: In situ hybridization of EBV-encoded small RNA-1 (EBER-1) and PCR was performed to identify the presence of EBV in GCs. Immunohistochemistry was used to identify expressions of bcl-2, bcl-xl, bak, bax, p53, NOS-1, NOS-2, and NOS-3 proteins in 25 EBV positive GCs and in 103 EBV negative GCS. Results: 12% of the cases of GC (25/208) showed EBER-1 and EBNA-1 expression. The cases were preferentially of diffuse type with intense lymphoid infiltrate in the stroma. EBV associated GCs showed higher expression of bcl-2 protein and lower expression of bak protein than in EBV negative GCs. Indeed, expressions of NOS-1 and NOS-3 were frequently observed in EBV associated GCs. Conclusion: Our data suggest that EBV infection may protect tumor cells from apoptosis, giving them the capacity for permanent cell cycling and proliferation. In addition, EBV positive GCs show high expression of constitutive NOS that could influence tumor progression and aggressiveness.
AB - Aim: To determine the incidence of Epstein Barr virus associated gastric carcinoma (GC) in Brazil and compare the expressions of apoptosis related proteins and nitric oxide synthases between EBV positive and negative gastric carcinoma. Methods: In situ hybridization of EBV-encoded small RNA-1 (EBER-1) and PCR was performed to identify the presence of EBV in GCs. Immunohistochemistry was used to identify expressions of bcl-2, bcl-xl, bak, bax, p53, NOS-1, NOS-2, and NOS-3 proteins in 25 EBV positive GCs and in 103 EBV negative GCS. Results: 12% of the cases of GC (25/208) showed EBER-1 and EBNA-1 expression. The cases were preferentially of diffuse type with intense lymphoid infiltrate in the stroma. EBV associated GCs showed higher expression of bcl-2 protein and lower expression of bak protein than in EBV negative GCs. Indeed, expressions of NOS-1 and NOS-3 were frequently observed in EBV associated GCs. Conclusion: Our data suggest that EBV infection may protect tumor cells from apoptosis, giving them the capacity for permanent cell cycling and proliferation. In addition, EBV positive GCs show high expression of constitutive NOS that could influence tumor progression and aggressiveness.
KW - Apoptosis
KW - EBV
KW - Gastric carcinoma
KW - Nitric oxide synthases
KW - P53
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U2 - 10.3748/wjg.v12.i31.4959
DO - 10.3748/wjg.v12.i31.4959
M3 - Article
C2 - 16937490
AN - SCOPUS:33748303089
SN - 1007-9327
VL - 12
SP - 4959
EP - 4965
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 31
ER -