Differential expression of apoptosis related proteins and nitric oxide synthases in Epstein Barr associated gastric carcinomas

Maria D. Begnami, Andre L. Montagnini, Andre L. Vettore, Sueli Nonogaki, Mariana Brait, Alex Y. Simoes-Sato, Andre Q.A. Seixas, Fernando A. Soares

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To determine the incidence of Epstein Barr virus associated gastric carcinoma (GC) in Brazil and compare the expressions of apoptosis related proteins and nitric oxide synthases between EBV positive and negative gastric carcinoma. Methods: In situ hybridization of EBV-encoded small RNA-1 (EBER-1) and PCR was performed to identify the presence of EBV in GCs. Immunohistochemistry was used to identify expressions of bcl-2, bcl-xl, bak, bax, p53, NOS-1, NOS-2, and NOS-3 proteins in 25 EBV positive GCs and in 103 EBV negative GCS. Results: 12% of the cases of GC (25/208) showed EBER-1 and EBNA-1 expression. The cases were preferentially of diffuse type with intense lymphoid infiltrate in the stroma. EBV associated GCs showed higher expression of bcl-2 protein and lower expression of bak protein than in EBV negative GCs. Indeed, expressions of NOS-1 and NOS-3 were frequently observed in EBV associated GCs. Conclusion: Our data suggest that EBV infection may protect tumor cells from apoptosis, giving them the capacity for permanent cell cycling and proliferation. In addition, EBV positive GCs show high expression of constitutive NOS that could influence tumor progression and aggressiveness.

Original languageEnglish (US)
Pages (from-to)4959-4965
Number of pages7
JournalWorld Journal of Gastroenterology
Volume12
Issue number31
DOIs
StatePublished - Aug 21 2006
Externally publishedYes

Keywords

  • Apoptosis
  • EBV
  • Gastric carcinoma
  • Nitric oxide synthases
  • P53

ASJC Scopus subject areas

  • Gastroenterology

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