Differential effects of TGF-β1 and TGF-β3 on chondrogenesis in posterofrontal cranial suture-derived mesenchymal cells in vitro

Aaron James, Yue Xu, Jacqueline K. Lee, Ruidi Wang, Michael T. Longaker

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Transforming growth factor (TGF)-β1 has been associated with cranial suture fusion, whereas TGF-β3 has been associated with suture patency. The mouse posterofrontal suture, analogous to the human metopic suture, fuses through endochondral ossification. METHODS: TGF-β1 and TGF-β3 expression in the posterofrontal suture was examined by immunohistochemistry. Next, the authors established cultures of suture-derived mesenchymal cells from the posterofrontal suture and examined the cellular responses to TGF-β1 and TGF-β3. Proliferation in response to TGF-β isoforms was examined by bromodeoxyuridine incorporation. High-density micromass culture of posterofrontal mesenchymal cells was used to study the effect of TGF-β1 and TGF-β3 on chondrogenic differentiation. RESULTS: TGF-β1 but not TGF-β3 protein was highly expressed in chondrocytes within the posterofrontal suture. Significant increases in posterofrontal cell proliferation were observed with TGF-β3 but not TGF-β1. TGF-β1 led to significant increases in chondrogenic-specific gene expression (including Sox9, Col II, Aggrecan, and Col X) as compared with moderate effects of TGF-β3. TGF-β1 increased cellular adhesion molecule expression (N-cadherin and fibronectin) and promoted cellular condensation, whereas TGF-β3 increased cellular proliferation (PCNA expression). Finally, TGF-β1 and, to a lesser extent, TGF-β3 induced the expression of fibroblast growth factors (FGF-2 and FGF-18). CONCLUSIONS: TGF-β1 and TGF-β3 exhibit marked differences in their effects on chondrogenesis in posterfrontal suture-derived mesenchymal cells, influencing different stages of chondrogenic differentiation. TGF-β3 significantly increased cellular proliferation, whereas TGF-β1 induced precartilage condensation, promoting chondrocyte differentiation.

Original languageEnglish (US)
Pages (from-to)31-43
Number of pages13
JournalPlastic and Reconstructive Surgery
Volume123
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

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Cranial Sutures
Chondrogenesis
Transforming Growth Factors
Sutures
In Vitro Techniques
Cell Proliferation
Fibroblast Growth Factor 2
Chondrocytes

ASJC Scopus subject areas

  • Surgery

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Differential effects of TGF-β1 and TGF-β3 on chondrogenesis in posterofrontal cranial suture-derived mesenchymal cells in vitro. / James, Aaron; Xu, Yue; Lee, Jacqueline K.; Wang, Ruidi; Longaker, Michael T.

In: Plastic and Reconstructive Surgery, Vol. 123, No. 1, 01.2009, p. 31-43.

Research output: Contribution to journalArticle

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abstract = "BACKGROUND: Transforming growth factor (TGF)-β1 has been associated with cranial suture fusion, whereas TGF-β3 has been associated with suture patency. The mouse posterofrontal suture, analogous to the human metopic suture, fuses through endochondral ossification. METHODS: TGF-β1 and TGF-β3 expression in the posterofrontal suture was examined by immunohistochemistry. Next, the authors established cultures of suture-derived mesenchymal cells from the posterofrontal suture and examined the cellular responses to TGF-β1 and TGF-β3. Proliferation in response to TGF-β isoforms was examined by bromodeoxyuridine incorporation. High-density micromass culture of posterofrontal mesenchymal cells was used to study the effect of TGF-β1 and TGF-β3 on chondrogenic differentiation. RESULTS: TGF-β1 but not TGF-β3 protein was highly expressed in chondrocytes within the posterofrontal suture. Significant increases in posterofrontal cell proliferation were observed with TGF-β3 but not TGF-β1. TGF-β1 led to significant increases in chondrogenic-specific gene expression (including Sox9, Col II, Aggrecan, and Col X) as compared with moderate effects of TGF-β3. TGF-β1 increased cellular adhesion molecule expression (N-cadherin and fibronectin) and promoted cellular condensation, whereas TGF-β3 increased cellular proliferation (PCNA expression). Finally, TGF-β1 and, to a lesser extent, TGF-β3 induced the expression of fibroblast growth factors (FGF-2 and FGF-18). CONCLUSIONS: TGF-β1 and TGF-β3 exhibit marked differences in their effects on chondrogenesis in posterfrontal suture-derived mesenchymal cells, influencing different stages of chondrogenic differentiation. TGF-β3 significantly increased cellular proliferation, whereas TGF-β1 induced precartilage condensation, promoting chondrocyte differentiation.",
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N2 - BACKGROUND: Transforming growth factor (TGF)-β1 has been associated with cranial suture fusion, whereas TGF-β3 has been associated with suture patency. The mouse posterofrontal suture, analogous to the human metopic suture, fuses through endochondral ossification. METHODS: TGF-β1 and TGF-β3 expression in the posterofrontal suture was examined by immunohistochemistry. Next, the authors established cultures of suture-derived mesenchymal cells from the posterofrontal suture and examined the cellular responses to TGF-β1 and TGF-β3. Proliferation in response to TGF-β isoforms was examined by bromodeoxyuridine incorporation. High-density micromass culture of posterofrontal mesenchymal cells was used to study the effect of TGF-β1 and TGF-β3 on chondrogenic differentiation. RESULTS: TGF-β1 but not TGF-β3 protein was highly expressed in chondrocytes within the posterofrontal suture. Significant increases in posterofrontal cell proliferation were observed with TGF-β3 but not TGF-β1. TGF-β1 led to significant increases in chondrogenic-specific gene expression (including Sox9, Col II, Aggrecan, and Col X) as compared with moderate effects of TGF-β3. TGF-β1 increased cellular adhesion molecule expression (N-cadherin and fibronectin) and promoted cellular condensation, whereas TGF-β3 increased cellular proliferation (PCNA expression). Finally, TGF-β1 and, to a lesser extent, TGF-β3 induced the expression of fibroblast growth factors (FGF-2 and FGF-18). CONCLUSIONS: TGF-β1 and TGF-β3 exhibit marked differences in their effects on chondrogenesis in posterfrontal suture-derived mesenchymal cells, influencing different stages of chondrogenic differentiation. TGF-β3 significantly increased cellular proliferation, whereas TGF-β1 induced precartilage condensation, promoting chondrocyte differentiation.

AB - BACKGROUND: Transforming growth factor (TGF)-β1 has been associated with cranial suture fusion, whereas TGF-β3 has been associated with suture patency. The mouse posterofrontal suture, analogous to the human metopic suture, fuses through endochondral ossification. METHODS: TGF-β1 and TGF-β3 expression in the posterofrontal suture was examined by immunohistochemistry. Next, the authors established cultures of suture-derived mesenchymal cells from the posterofrontal suture and examined the cellular responses to TGF-β1 and TGF-β3. Proliferation in response to TGF-β isoforms was examined by bromodeoxyuridine incorporation. High-density micromass culture of posterofrontal mesenchymal cells was used to study the effect of TGF-β1 and TGF-β3 on chondrogenic differentiation. RESULTS: TGF-β1 but not TGF-β3 protein was highly expressed in chondrocytes within the posterofrontal suture. Significant increases in posterofrontal cell proliferation were observed with TGF-β3 but not TGF-β1. TGF-β1 led to significant increases in chondrogenic-specific gene expression (including Sox9, Col II, Aggrecan, and Col X) as compared with moderate effects of TGF-β3. TGF-β1 increased cellular adhesion molecule expression (N-cadherin and fibronectin) and promoted cellular condensation, whereas TGF-β3 increased cellular proliferation (PCNA expression). Finally, TGF-β1 and, to a lesser extent, TGF-β3 induced the expression of fibroblast growth factors (FGF-2 and FGF-18). CONCLUSIONS: TGF-β1 and TGF-β3 exhibit marked differences in their effects on chondrogenesis in posterfrontal suture-derived mesenchymal cells, influencing different stages of chondrogenic differentiation. TGF-β3 significantly increased cellular proliferation, whereas TGF-β1 induced precartilage condensation, promoting chondrocyte differentiation.

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