Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms

Athanasios Chatzimoschou, Maria Simitsopoulou, Charalampos Antachopoulos, Thomas J. Walsh, Emmanuel Roilides

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to analyse specific molecular mechanisms involved in the intrinsic resistance of C. albicans biofilms to antifungals. We investigated the transcriptional profile of three genes (BGL2, SUN41, ECE1) involved in Candida cell wall formation in response to voriconazole or anidulafungin after the production of intermediate and mature biofilms. C. albicans M61, a well-documented biofilm producer strain, was used for the development of intermediate (12 h and 18 h) and completely mature biofilms (48 h). After exposure of cells from each biofilm growth mode to voriconazole (128 and 512 mg l-1) or anidulafungin (0.25 and 1 mg l-1) for 12-24 h, total RNA samples extracted from biofilm cells were analysed by RT-PCR. The voriconazole and anidulafungin biofilm MIC was 512 and 0.5 mg l-1 respectively. Anidulafungin caused significant up-regulation of SUN41 (3.7-9.3-fold) and BGL2 (2.2-2.8 fold) in intermediately mature biofilms; whereas, voriconazole increased gene expression in completely mature biofilms (SUN41 2.3-fold, BGL2 2.1-fold). Gene expression was primarily down-regulated by voriconazole in intermediately, but not completely mature biofilms. Both antifungals caused down-regulation of ECE1 in intermediately mature biofilms.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalMycoses
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Antifungal Agents
Biofilms
Candida albicans
anidulafungin
Gene Expression
Candida
Cell Wall
Up-Regulation
Down-Regulation
Voriconazole
RNA

Keywords

  • C. albicans
  • Anidulafungin
  • Biofilms
  • Gene expression
  • Voriconazole

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Chatzimoschou, A., Simitsopoulou, M., Antachopoulos, C., Walsh, T. J., & Roilides, E. (2016). Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms. Mycoses, 59(1), 43-47. https://doi.org/10.1111/myc.12436

Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms. / Chatzimoschou, Athanasios; Simitsopoulou, Maria; Antachopoulos, Charalampos; Walsh, Thomas J.; Roilides, Emmanuel.

In: Mycoses, Vol. 59, No. 1, 01.01.2016, p. 43-47.

Research output: Contribution to journalArticle

Chatzimoschou, A, Simitsopoulou, M, Antachopoulos, C, Walsh, TJ & Roilides, E 2016, 'Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms', Mycoses, vol. 59, no. 1, pp. 43-47. https://doi.org/10.1111/myc.12436
Chatzimoschou, Athanasios ; Simitsopoulou, Maria ; Antachopoulos, Charalampos ; Walsh, Thomas J. ; Roilides, Emmanuel. / Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms. In: Mycoses. 2016 ; Vol. 59, No. 1. pp. 43-47.
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