Differential developmental deficits in retinal function in the absence of either protein Tyrosine Sulfotransferase-1 or -2

David M. Sherry, Yogita Kanan, Robert Hamilton, Adam Hoffhines, Kelsey L. Arbogast, Steven J. Fliesler, Muna I. Naash, Kevin L. Moore, Muayyad R. Al-Ubaidi

Research output: Contribution to journalArticle

Abstract

To investigate the role(s) of protein-tyrosine sulfation in the retina and to determine the differential role(s) of tyrosylprotein sulfotransferases (TPST) 1 and 2 in vision, retinal function and structure were examined in mice lacking TPST-1 or TPST-2. Despite the normal histologic retinal appearance in both Tpst1-/- and Tpst2-/- mice, retinal function was compromised during early development. However, Tpst1-/- retinas became electrophysiologically normal by postnatal day 90 while Tpst2-/- mice did not functionally normalize with age. Ultrastructurally, the absence of TPST-1 or TPST-2 caused minor reductions in neuronal plexus. These results demonstrate the functional importance of protein-tyrosine sulfation for proper development of the retina and suggest that the different phenotypes resulting from elimination of either TPST-1 or -2 may reflect differential expression patterns or levels of the enzymes. Furthermore, single knock-out mice of either TPST-1 or -2 did not phenocopy mice with double-knockout of both TPSTs, suggesting that the functions of the TPSTs are at least partially redundant, which points to the functional importance of these enzymes in the retina.

Original languageEnglish (US)
Article numbere39702
JournalPLoS One
Volume7
Issue number6
DOIs
StatePublished - Jun 22 2012
Externally publishedYes

Fingerprint

sulfotransferases
tyrosine
retina
Retina
mice
proteins
Tyrosine
plexus
Enzymes
enzymes
protein-tyrosine sulfotransferase
Knockout Mice
early development
Proteins
Phenotype
phenotype

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sherry, D. M., Kanan, Y., Hamilton, R., Hoffhines, A., Arbogast, K. L., Fliesler, S. J., ... Al-Ubaidi, M. R. (2012). Differential developmental deficits in retinal function in the absence of either protein Tyrosine Sulfotransferase-1 or -2. PLoS One, 7(6), [e39702]. https://doi.org/10.1371/journal.pone.0039702

Differential developmental deficits in retinal function in the absence of either protein Tyrosine Sulfotransferase-1 or -2. / Sherry, David M.; Kanan, Yogita; Hamilton, Robert; Hoffhines, Adam; Arbogast, Kelsey L.; Fliesler, Steven J.; Naash, Muna I.; Moore, Kevin L.; Al-Ubaidi, Muayyad R.

In: PLoS One, Vol. 7, No. 6, e39702, 22.06.2012.

Research output: Contribution to journalArticle

Sherry, DM, Kanan, Y, Hamilton, R, Hoffhines, A, Arbogast, KL, Fliesler, SJ, Naash, MI, Moore, KL & Al-Ubaidi, MR 2012, 'Differential developmental deficits in retinal function in the absence of either protein Tyrosine Sulfotransferase-1 or -2', PLoS One, vol. 7, no. 6, e39702. https://doi.org/10.1371/journal.pone.0039702
Sherry, David M. ; Kanan, Yogita ; Hamilton, Robert ; Hoffhines, Adam ; Arbogast, Kelsey L. ; Fliesler, Steven J. ; Naash, Muna I. ; Moore, Kevin L. ; Al-Ubaidi, Muayyad R. / Differential developmental deficits in retinal function in the absence of either protein Tyrosine Sulfotransferase-1 or -2. In: PLoS One. 2012 ; Vol. 7, No. 6.
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