Differential denaturation of Haemophilus influenzae DNA

Alan D. Cooper; P. C. Huang

doi:10.1016/0005-2787(71)90575-2

Differential denaturation of Haemophilus influenzae DNA

Alan D. Cooper, P. C. Huang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The objective of this study is to determine whether differential denaturation of various genes can be observed. By treatments with heat or by titration with hydroxyl ions at subcritical levels, genetic markers from Haemophilus influenzae have been inactivated in the order of streptomycin resistance > erythromycin resistance > novobiocin resistance. The kinetics and extent of inactivation depends on whether a thermal or alkaline method is used. Results obtained with an assay system which allows the expression of single-stranded DNA are complementary to those testing the transforming activity of double-stranded DNA.

Original language	English (US)
Pages (from-to)	234-245
Number of pages	12
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	232
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(71)90575-2
State	Published - Mar 11 1971

ASJC Scopus subject areas

General Medicine

Access to Document

10.1016/0005-2787(71)90575-2

Cite this

@article{356a03eb3280458c8f62e5138690a9e9,

title = "Differential denaturation of Haemophilus influenzae DNA",

abstract = "The objective of this study is to determine whether differential denaturation of various genes can be observed. By treatments with heat or by titration with hydroxyl ions at subcritical levels, genetic markers from Haemophilus influenzae have been inactivated in the order of streptomycin resistance > erythromycin resistance > novobiocin resistance. The kinetics and extent of inactivation depends on whether a thermal or alkaline method is used. Results obtained with an assay system which allows the expression of single-stranded DNA are complementary to those testing the transforming activity of double-stranded DNA.",

author = "Cooper, {Alan D.} and Huang, {P. C.}",

note = "Funding Information: The work is supported in part by a National Institute of Health, U.S. Public Health Service Postdoctoral fellowship to A. D. C. (FRo5445), and Research Career Development Award to P. C. H. (I-K3GM31-238-oI) and by a grant from National Science Foundation (GB 8058). We are grateful to William M. Swartz and Charles Braslow who have contributed, respectively, in defining some of the experimental conditions for thermal and alkaline denaturation reported here. We thank Dr. Roger M. Herriott for his encouragement.",

year = "1971",

month = mar,

day = "11",

doi = "10.1016/0005-2787(71)90575-2",

language = "English (US)",

volume = "232",

pages = "234--245",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

issn = "0005-2787",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Differential denaturation of Haemophilus influenzae DNA

AU - Cooper, Alan D.

AU - Huang, P. C.

N1 - Funding Information: The work is supported in part by a National Institute of Health, U.S. Public Health Service Postdoctoral fellowship to A. D. C. (FRo5445), and Research Career Development Award to P. C. H. (I-K3GM31-238-oI) and by a grant from National Science Foundation (GB 8058). We are grateful to William M. Swartz and Charles Braslow who have contributed, respectively, in defining some of the experimental conditions for thermal and alkaline denaturation reported here. We thank Dr. Roger M. Herriott for his encouragement.

PY - 1971/3/11

Y1 - 1971/3/11

N2 - The objective of this study is to determine whether differential denaturation of various genes can be observed. By treatments with heat or by titration with hydroxyl ions at subcritical levels, genetic markers from Haemophilus influenzae have been inactivated in the order of streptomycin resistance > erythromycin resistance > novobiocin resistance. The kinetics and extent of inactivation depends on whether a thermal or alkaline method is used. Results obtained with an assay system which allows the expression of single-stranded DNA are complementary to those testing the transforming activity of double-stranded DNA.

AB - The objective of this study is to determine whether differential denaturation of various genes can be observed. By treatments with heat or by titration with hydroxyl ions at subcritical levels, genetic markers from Haemophilus influenzae have been inactivated in the order of streptomycin resistance > erythromycin resistance > novobiocin resistance. The kinetics and extent of inactivation depends on whether a thermal or alkaline method is used. Results obtained with an assay system which allows the expression of single-stranded DNA are complementary to those testing the transforming activity of double-stranded DNA.

UR - http://www.scopus.com/inward/record.url?scp=0015219419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015219419&partnerID=8YFLogxK

U2 - 10.1016/0005-2787(71)90575-2

DO - 10.1016/0005-2787(71)90575-2

M3 - Article

C2 - 5313787

AN - SCOPUS:0015219419

SN - 0005-2787

VL - 232

SP - 234

EP - 245

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 2

ER -

Differential denaturation of Haemophilus influenzae DNA

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this