Differential binding properties of B7-H1 and B7-DC to programmed death-1

Pornpan Youngnak, Yuko Kozono, Haruo Kozono, Hideyuki Iwai, Noriko Otsuki, Hisayo Jin, Ken Omura, Hideo Yagita, Drew M. Pardoll, Lieping Chen, Miyuki Azuma

Research output: Contribution to journalArticle

Abstract

Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.

Original languageEnglish (US)
Pages (from-to)672-677
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume307
Issue number3
DOIs
StatePublished - Aug 1 2003

Keywords

  • Costimulatory molecule
  • PD-1
  • PD-1 ligands
  • Relative affinity
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Youngnak, P., Kozono, Y., Kozono, H., Iwai, H., Otsuki, N., Jin, H., Omura, K., Yagita, H., Pardoll, D. M., Chen, L., & Azuma, M. (2003). Differential binding properties of B7-H1 and B7-DC to programmed death-1. Biochemical and Biophysical Research Communications, 307(3), 672-677. https://doi.org/10.1016/S0006-291X(03)01257-9