Differential binding properties of B7-H1 and B7-DC to programmed death-1

Pornpan Youngnak; Yuko Kozono; Haruo Kozono; Hideyuki Iwai; Noriko Otsuki; Hisayo Jin; Ken Omura; Hideo Yagita; Drew M. Pardoll; Lieping Chen; Miyuki Azuma

doi:10.1016/S0006-291X(03)01257-9

Differential binding properties of B7-H1 and B7-DC to programmed death-1

Pornpan Youngnak, Yuko Kozono, Haruo Kozono, Hideyuki Iwai, Noriko Otsuki, Hisayo Jin, Ken Omura, Hideo Yagita, Drew M. Pardoll, Lieping Chen, Miyuki Azuma

School of Medicine

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.

Original language	English (US)
Pages (from-to)	672-677
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	307
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(03)01257-9
State	Published - Aug 1 2003

Keywords

Costimulatory molecule
PD-1
PD-1 ligands
Relative affinity
Surface plasmon resonance

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(03)01257-9

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title = "Differential binding properties of B7-H1 and B7-DC to programmed death-1",

abstract = "Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.",

keywords = "Costimulatory molecule, PD-1, PD-1 ligands, Relative affinity, Surface plasmon resonance",

author = "Pornpan Youngnak and Yuko Kozono and Haruo Kozono and Hideyuki Iwai and Noriko Otsuki and Hisayo Jin and Ken Omura and Hideo Yagita and Pardoll, {Drew M.} and Lieping Chen and Miyuki Azuma",

note = "Funding Information: This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, and from the Ministry of Health, Labor, and Welfare of Japan.",

year = "2003",

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doi = "10.1016/S0006-291X(03)01257-9",

language = "English (US)",

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T1 - Differential binding properties of B7-H1 and B7-DC to programmed death-1

AU - Youngnak, Pornpan

AU - Kozono, Yuko

AU - Kozono, Haruo

AU - Iwai, Hideyuki

AU - Otsuki, Noriko

AU - Jin, Hisayo

AU - Omura, Ken

AU - Yagita, Hideo

AU - Pardoll, Drew M.

AU - Chen, Lieping

AU - Azuma, Miyuki

N1 - Funding Information: This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, and from the Ministry of Health, Labor, and Welfare of Japan.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.

AB - Programmed death-1 (PD-1) is a negative regulatory receptor expressed on activated T and B cells. Two ligands for PD-1, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified, but their binding properties have not been characterized yet. In this study, we generated soluble Ig fusion proteins of these molecules and examined the kinetics and relative affinities of the interactions between B7-H1 or B7-DC and PD-1 by flow cytometry and surface plasmon resonance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity and had different association/dissociation kinetics compared with the interaction of B7-H1/PD-1. Our results suggest that the differential binding properties of B7-H1 and B7-DC may be responsible for differential contributions of these two PD-1 ligands to immune responses.

KW - Costimulatory molecule

KW - PD-1

KW - PD-1 ligands

KW - Relative affinity

KW - Surface plasmon resonance

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Differential binding properties of B7-H1 and B7-DC to programmed death-1

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Keywords

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