Different specificities of cloned Τ cells assessed by in vitro proliferation assays and by the ability to mediate skin graft rejection in vivo

David H. Lynch, Douglas J. Weiland, Steven A. Rosenberg, Richard J. Hodes

Research output: Contribution to journalArticle

Abstract

The experiments presented here have compared the specificities of T cell clones as determined by in vitro proliferative responses and their specificities as reflected by their ability to mediate skin graft rejection in vivo. Two proliferative T cell clones with distinct in vitro specificities were evaluated for their ability to mediate rejection of skin grafts from C57BL/10 Sen nu/nu mice. Clone 14.11 (L3T4+, Lyt2) was specifically stimulated to proliferate in vitro by I-Ad determinants, while clone 3.3.10 (also L3T4+, Lyt2) recognized M1sa products in the context of МНC-encoded cell surface determinants. The results demonstrate that both clone 14.11 and clone 3.3.10 T cells are capable of mediating rejection of DBA/2 (H-2d, M1sa) skin grafts from B10 nu/nu mice. Surprisingly, neither clone 3.3.10 T cells nor clone 14.11 T cells were found to be effective at rejecting skin grafts from the D1.C congenie donor strain of mice (also H-2d, M1sa) from BIO nu/nu mice. Further, clone 14.11 T cells were also found to be ineffective at rejecting B10.D2 (Н-2d, M1sb) skin grafts from B10 nu/nu mice. These data indicate the existence of a striking dichotomy between the specificity of alloreactive T cell clones as determined by in vitro proliferation studies and their functional capabilities in vivo.

Original languageEnglish (US)
Pages (from-to)408-411
Number of pages4
JournalTransplantation
Volume43
Issue number3
DOIs
StatePublished - Mar 1987

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ASJC Scopus subject areas

  • Transplantation

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