TY - JOUR
T1 - Different development of myelin basic protein agonist- and antagonist-specific human TCR transgenic T cells in the thymus and periphery
AU - Kawamura, Kazuyuki
AU - Yao, Karen
AU - Shukaliak-Quandt, Jacqueline A.
AU - Huh, Jaebong
AU - Baig, Mirza
AU - Quigley, Laura
AU - Ito, Naoko
AU - Necker, Antje
AU - McFarland, Henry F.
AU - Muraro, Paolo A.
AU - Martin, Roland
AU - Ito, Kouichi
PY - 2008
Y1 - 2008
N2 - Myelin basic protein (MBP)-specific T cells are thought to play a role in the development of multiple sclerosis. MBP residues 111-129 compose an immunodominant epitope cluster restricted by HLA-DRB1*0401. The sequence of residues 111-129 of MBP (MBP111-129) differs in humans (MBP122:Arg) and mice (MBP122:Lys) at aa 122. We previously found that ∼50% of human MBP111-129 (MBP122: Arg)-specific T cell clones, including MS2-3C8 can proliferate in response to mouse MBP111-129 (MBP122:Lys). However, the other half of T cell clones, including HD4-1C2, cannot proliferate in response to MBP111-129 (MBP122:Lys). We found that MBP111-129 (MBP122: Lys) is an antagonist for HD4-1C2 TCR, therefore, MS2-3C8 and HD4-1C2 TCRs are agonist- and antagonist-specific TCRs in mice, respectively. Therefore, we examined the development of HD4-1C2 TCR and MS2-3C8 TCR transgenic (Tg) T cells in the thymus and periphery. We found that dual TCR expression exclusively facilitates the development of MBP111-129 TCR Tg T cells in the periphery of HD4-1C2 TCR/HLA-DRB1*0401 Tg mice although it is not required for their development in the thymus. We also found that MS2-3C8 TCR Tg CD8 + T cells develop along with MS2-3C8 TCR Tg CD4 T cells, and that dual TCR expression was crucial for the development of MS2-3C8 TCR Tg CD4 + and CD8+ T cells in the thymus and periphery, respectively. These results suggest that thymic and peripheral development of MBP-specific T cells are different; however, dual TCR expression can facilitate their development.
AB - Myelin basic protein (MBP)-specific T cells are thought to play a role in the development of multiple sclerosis. MBP residues 111-129 compose an immunodominant epitope cluster restricted by HLA-DRB1*0401. The sequence of residues 111-129 of MBP (MBP111-129) differs in humans (MBP122:Arg) and mice (MBP122:Lys) at aa 122. We previously found that ∼50% of human MBP111-129 (MBP122: Arg)-specific T cell clones, including MS2-3C8 can proliferate in response to mouse MBP111-129 (MBP122:Lys). However, the other half of T cell clones, including HD4-1C2, cannot proliferate in response to MBP111-129 (MBP122:Lys). We found that MBP111-129 (MBP122: Lys) is an antagonist for HD4-1C2 TCR, therefore, MS2-3C8 and HD4-1C2 TCRs are agonist- and antagonist-specific TCRs in mice, respectively. Therefore, we examined the development of HD4-1C2 TCR and MS2-3C8 TCR transgenic (Tg) T cells in the thymus and periphery. We found that dual TCR expression exclusively facilitates the development of MBP111-129 TCR Tg T cells in the periphery of HD4-1C2 TCR/HLA-DRB1*0401 Tg mice although it is not required for their development in the thymus. We also found that MS2-3C8 TCR Tg CD8 + T cells develop along with MS2-3C8 TCR Tg CD4 T cells, and that dual TCR expression was crucial for the development of MS2-3C8 TCR Tg CD4 + and CD8+ T cells in the thymus and periphery, respectively. These results suggest that thymic and peripheral development of MBP-specific T cells are different; however, dual TCR expression can facilitate their development.
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M3 - Article
C2 - 18832703
AN - SCOPUS:54049145487
SN - 0022-1767
VL - 181
SP - 5462
EP - 5472
JO - Journal of Immunology
JF - Journal of Immunology
IS - 8
ER -