Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia

Wenju Lu, Jian Wang, Larissa Shimoda, J. T. Sylvester

Research output: Contribution to journalArticle

Abstract

Hypoxic pulmonary vasoconstriction (HPV) requires Ca2+ influx through store-operated Ca2+ channels (SOCC) in pulmonary arterial smooth muscle cells (PASMC) and is greater in distal than proximal pulmonary arteries (PA). SOCC may be composed of canonical transient receptor potential (TRPC) proteins and activated by stromal interacting molecule 1 (STIM1). To assess the possibility that HPV is greater in distal PA because store-operated Ca2+ entry (SOCE) is greater in distal PASMC, we measured intracellular Ca2+ concentration ([Ca2+]i) and SOCE in primary cultures of PASMC using fluorescent microscopy and the Ca 2+-sensitive dye fura 2. Both hypoxia (4% O2) and KCl (60 mM) increased [Ca2+]i. Responses to hypoxia, but not KCl, were greater in distal cells. We measured SOCE in PASMC perfused with Ca 2+-free solutions containing cyclopiazonic acid to deplete Ca 2+ stores in sarcoplasmic reticulum and nifedipine to prevent Ca 2+ entry through L-type voltage-operated Ca2+ channels. Under these conditions, the increase in [Ca2+]i caused by restoration of extracellular Ca2+ and the decrease in fura 2 fluorescence caused by Mn2+ were greater in distal PASMC, indicating greater SOCE. Moreover, the increase in SOCE caused by hypoxia was also greater in distal cells. Real-time quantitative polymerase chain reaction analysis of PASMC and freshly isolated deendothelialized PA tissue demonstrated expression of STIM1 and five of seven known TRPC isoforms (TRPC1 > TRPC6 > TRPC4 ≫ TRPC3 ≈ TRPC5). For both protein, as measured by Western blotting, and mRNA, expression of STIM1, TRPC1, TRPC6, and TRPC4 was greater in distal than proximal PASMC and PA. These results provide further support for the importance of SOCE in HPV and suggest that HPV is greater in distal than proximal PA because greater numbers and activation of SOCC in distal PASMC generate bigger increases in [Ca2+]i.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume295
Issue number1
DOIs
StatePublished - Jul 2008

Fingerprint

Smooth Muscle
Lung
Smooth Muscle Myocytes
Pulmonary Artery
Vasoconstriction
Fura-2
Hypoxia
Sarcoplasmic Reticulum
Nifedipine
Real-Time Polymerase Chain Reaction
Microscopy
Protein Isoforms
Proteins
Fluorescence
Western Blotting
Messenger RNA

Keywords

  • Calcium ion
  • Calcium signaling
  • Canonical transient receptor potential
  • Hypoxic pulmonary vasoconstriction
  • Potassium chloride
  • Stromal interacting molecule 1
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

@article{5e91ea5140dc4b5e8ae62ade92eebfc1,
title = "Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia",
abstract = "Hypoxic pulmonary vasoconstriction (HPV) requires Ca2+ influx through store-operated Ca2+ channels (SOCC) in pulmonary arterial smooth muscle cells (PASMC) and is greater in distal than proximal pulmonary arteries (PA). SOCC may be composed of canonical transient receptor potential (TRPC) proteins and activated by stromal interacting molecule 1 (STIM1). To assess the possibility that HPV is greater in distal PA because store-operated Ca2+ entry (SOCE) is greater in distal PASMC, we measured intracellular Ca2+ concentration ([Ca2+]i) and SOCE in primary cultures of PASMC using fluorescent microscopy and the Ca 2+-sensitive dye fura 2. Both hypoxia (4{\%} O2) and KCl (60 mM) increased [Ca2+]i. Responses to hypoxia, but not KCl, were greater in distal cells. We measured SOCE in PASMC perfused with Ca 2+-free solutions containing cyclopiazonic acid to deplete Ca 2+ stores in sarcoplasmic reticulum and nifedipine to prevent Ca 2+ entry through L-type voltage-operated Ca2+ channels. Under these conditions, the increase in [Ca2+]i caused by restoration of extracellular Ca2+ and the decrease in fura 2 fluorescence caused by Mn2+ were greater in distal PASMC, indicating greater SOCE. Moreover, the increase in SOCE caused by hypoxia was also greater in distal cells. Real-time quantitative polymerase chain reaction analysis of PASMC and freshly isolated deendothelialized PA tissue demonstrated expression of STIM1 and five of seven known TRPC isoforms (TRPC1 > TRPC6 > TRPC4 ≫ TRPC3 ≈ TRPC5). For both protein, as measured by Western blotting, and mRNA, expression of STIM1, TRPC1, TRPC6, and TRPC4 was greater in distal than proximal PASMC and PA. These results provide further support for the importance of SOCE in HPV and suggest that HPV is greater in distal than proximal PA because greater numbers and activation of SOCC in distal PASMC generate bigger increases in [Ca2+]i.",
keywords = "Calcium ion, Calcium signaling, Canonical transient receptor potential, Hypoxic pulmonary vasoconstriction, Potassium chloride, Stromal interacting molecule 1, Vascular smooth muscle",
author = "Wenju Lu and Jian Wang and Larissa Shimoda and Sylvester, {J. T.}",
year = "2008",
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language = "English (US)",
volume = "295",
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TY - JOUR

T1 - Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia

AU - Lu, Wenju

AU - Wang, Jian

AU - Shimoda, Larissa

AU - Sylvester, J. T.

PY - 2008/7

Y1 - 2008/7

N2 - Hypoxic pulmonary vasoconstriction (HPV) requires Ca2+ influx through store-operated Ca2+ channels (SOCC) in pulmonary arterial smooth muscle cells (PASMC) and is greater in distal than proximal pulmonary arteries (PA). SOCC may be composed of canonical transient receptor potential (TRPC) proteins and activated by stromal interacting molecule 1 (STIM1). To assess the possibility that HPV is greater in distal PA because store-operated Ca2+ entry (SOCE) is greater in distal PASMC, we measured intracellular Ca2+ concentration ([Ca2+]i) and SOCE in primary cultures of PASMC using fluorescent microscopy and the Ca 2+-sensitive dye fura 2. Both hypoxia (4% O2) and KCl (60 mM) increased [Ca2+]i. Responses to hypoxia, but not KCl, were greater in distal cells. We measured SOCE in PASMC perfused with Ca 2+-free solutions containing cyclopiazonic acid to deplete Ca 2+ stores in sarcoplasmic reticulum and nifedipine to prevent Ca 2+ entry through L-type voltage-operated Ca2+ channels. Under these conditions, the increase in [Ca2+]i caused by restoration of extracellular Ca2+ and the decrease in fura 2 fluorescence caused by Mn2+ were greater in distal PASMC, indicating greater SOCE. Moreover, the increase in SOCE caused by hypoxia was also greater in distal cells. Real-time quantitative polymerase chain reaction analysis of PASMC and freshly isolated deendothelialized PA tissue demonstrated expression of STIM1 and five of seven known TRPC isoforms (TRPC1 > TRPC6 > TRPC4 ≫ TRPC3 ≈ TRPC5). For both protein, as measured by Western blotting, and mRNA, expression of STIM1, TRPC1, TRPC6, and TRPC4 was greater in distal than proximal PASMC and PA. These results provide further support for the importance of SOCE in HPV and suggest that HPV is greater in distal than proximal PA because greater numbers and activation of SOCC in distal PASMC generate bigger increases in [Ca2+]i.

AB - Hypoxic pulmonary vasoconstriction (HPV) requires Ca2+ influx through store-operated Ca2+ channels (SOCC) in pulmonary arterial smooth muscle cells (PASMC) and is greater in distal than proximal pulmonary arteries (PA). SOCC may be composed of canonical transient receptor potential (TRPC) proteins and activated by stromal interacting molecule 1 (STIM1). To assess the possibility that HPV is greater in distal PA because store-operated Ca2+ entry (SOCE) is greater in distal PASMC, we measured intracellular Ca2+ concentration ([Ca2+]i) and SOCE in primary cultures of PASMC using fluorescent microscopy and the Ca 2+-sensitive dye fura 2. Both hypoxia (4% O2) and KCl (60 mM) increased [Ca2+]i. Responses to hypoxia, but not KCl, were greater in distal cells. We measured SOCE in PASMC perfused with Ca 2+-free solutions containing cyclopiazonic acid to deplete Ca 2+ stores in sarcoplasmic reticulum and nifedipine to prevent Ca 2+ entry through L-type voltage-operated Ca2+ channels. Under these conditions, the increase in [Ca2+]i caused by restoration of extracellular Ca2+ and the decrease in fura 2 fluorescence caused by Mn2+ were greater in distal PASMC, indicating greater SOCE. Moreover, the increase in SOCE caused by hypoxia was also greater in distal cells. Real-time quantitative polymerase chain reaction analysis of PASMC and freshly isolated deendothelialized PA tissue demonstrated expression of STIM1 and five of seven known TRPC isoforms (TRPC1 > TRPC6 > TRPC4 ≫ TRPC3 ≈ TRPC5). For both protein, as measured by Western blotting, and mRNA, expression of STIM1, TRPC1, TRPC6, and TRPC4 was greater in distal than proximal PASMC and PA. These results provide further support for the importance of SOCE in HPV and suggest that HPV is greater in distal than proximal PA because greater numbers and activation of SOCC in distal PASMC generate bigger increases in [Ca2+]i.

KW - Calcium ion

KW - Calcium signaling

KW - Canonical transient receptor potential

KW - Hypoxic pulmonary vasoconstriction

KW - Potassium chloride

KW - Stromal interacting molecule 1

KW - Vascular smooth muscle

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DO - 10.1152/ajplung.00058.2008

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VL - 295

JO - American Journal of Physiology

JF - American Journal of Physiology

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