TY - JOUR
T1 - Differences between human and mouse embryonic stem cells
AU - Ginis, Irene
AU - Luo, Yongquan
AU - Miura, Takumi
AU - Thies, Scott
AU - Brandenberger, Ralph
AU - Gerecht-Nir, Sharon
AU - Amit, Michal
AU - Hoke, Ahmet
AU - Carpenter, Melissa K.
AU - Itskovitz-Eldor, Joseph
AU - Rao, Mahendra S.
N1 - Funding Information:
We thank Dr. Mark Weiss for his valuable comments on this manuscript. We gratefully acknowledge the input of all members of our laboratory provided through discussions and constructive criticisms. Mahendra S. Rao was supported by the CNS foundation, NIA, NINDS and the Packard ALS center at Johns Hopkins University. Ahmet Hoke was supported by NINDS and the Packard ALS Center at Johns Hopkins University.
PY - 2004/5/15
Y1 - 2004/5/15
N2 - We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, β-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.
AB - We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, β-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.
KW - Apoptosis
KW - Cell cycle
KW - Cytokines
KW - Embryonic stem cells
KW - Human
KW - Leukemia inhibitory factor
KW - Markers
KW - Mouse
KW - RT-PCR
KW - cDNA microarray
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UR - http://www.scopus.com/inward/citedby.url?scp=11144357877&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2003.12.034
DO - 10.1016/j.ydbio.2003.12.034
M3 - Article
C2 - 15110706
AN - SCOPUS:11144357877
VL - 269
SP - 360
EP - 380
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -