Abstract
We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, β-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 360-380 |
| Number of pages | 21 |
| Journal | Developmental Biology |
| Volume | 269 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 15 2004 |
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Keywords
- Apoptosis
- cDNA microarray
- Cell cycle
- Cytokines
- Embryonic stem cells
- Human
- Leukemia inhibitory factor
- Markers
- Mouse
- RT-PCR
ASJC Scopus subject areas
- Developmental Biology
Cite this
Differences between human and mouse embryonic stem cells. / Ginis, Irene; Luo, Yongquan; Miura, Takumi; Thies, Scott; Brandenberger, Ralph; Gerecht-Nir, Sharon; Amit, Michal; Hoke, Ahmet; Carpenter, Melissa K.; Itskovitz-Eldor, Joseph; Rao, Mahendra S.
In: Developmental Biology, Vol. 269, No. 2, 15.05.2004, p. 360-380.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differences between human and mouse embryonic stem cells
AU - Ginis, Irene
AU - Luo, Yongquan
AU - Miura, Takumi
AU - Thies, Scott
AU - Brandenberger, Ralph
AU - Gerecht-Nir, Sharon
AU - Amit, Michal
AU - Hoke, Ahmet
AU - Carpenter, Melissa K.
AU - Itskovitz-Eldor, Joseph
AU - Rao, Mahendra S.
PY - 2004/5/15
Y1 - 2004/5/15
N2 - We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, β-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.
AB - We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, β-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.
KW - Apoptosis
KW - cDNA microarray
KW - Cell cycle
KW - Cytokines
KW - Embryonic stem cells
KW - Human
KW - Leukemia inhibitory factor
KW - Markers
KW - Mouse
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=11144357877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11144357877&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2003.12.034
DO - 10.1016/j.ydbio.2003.12.034
M3 - Article
C2 - 15110706
AN - SCOPUS:11144357877
VL - 269
SP - 360
EP - 380
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -