Dietary supplementation with docosahexaenoic acid, but not eicosapentaenoic acid, dramatically alters cardiac mitochondrial phospholipid fatty acid composition and prevents permeability transition

Ramzi J. Khairallah, Genevieve C. Sparagna, Nishanth Khanna, Karen M. O'Shea, Peter A. Hecker, Tibor Kristian, Gary Fiskum, Christine Des Rosiers, Brian M. Polster, William C. Stanley

Research output: Contribution to journalArticle

Abstract

Treatment with the ω-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) exerts cardioprotective effects, and suppresses Ca2+-induced opening of the mitochondrial permeability transition pore (MPTP). These effects are associated with increased DHA and EPA, and lower arachidonic acid (ARA) in cardiac phospholipids. While clinical studies suggest the triglyceride lowering effects of DHA and EPA are equivalent, little is known about the independent effects of DHA and EPA on mitochondria function. We compared the effects of dietary supplementation with the ω-3 PUFAs DHA and EPA on cardiac mitochondrial phospholipid fatty acid composition and Ca2+-induced MPTP opening. Rats were fed a standard lab diet with either normal low levels of ω-3 PUFA, or DHA or EPA at 2.5% of energy intake for 8weeks, and cardiac mitochondria were isolated and analyzed for Ca2+-induced MPTP opening and phospholipid fatty acyl composition. DHA supplementation increased both DHA and EPA and decreased ARA in mitochondrial phospholipid, and significantly delayed MPTP opening as assessed by increased Ca2+ retention capacity and decreased Ca2+-induced mitochondria swelling. EPA supplementation increased EPA in mitochondrial phospholipids, but did not affect DHA, only modestly lowered ARA, and did not affect MPTP opening. In summary, dietary supplementation with DHA but not EPA, profoundly altered mitochondrial phospholipid fatty acid composition and delayed Ca2+-induced MPTP opening.

Original languageEnglish (US)
Pages (from-to)1555-1562
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1797
Issue number8
DOIs
StatePublished - Aug 2010

Keywords

  • Cardiac
  • Docosahexaenoic acid
  • Eicosapentaenoic acid
  • Fish oil
  • Heart
  • Mitochondrial Permeability transition pore

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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