Dietary Intake of Aflatoxins and the Level of Albumin-bound Aflatoxin in Peripheral Blood in The Gambia, West Africa

Christopher P. Wild, Brigitte Chapot, Andrew J. Hall, Ruggero Montesano, Geoffrey J. Hudson, Gabriele Sabbioni, Gerald N. Wogan, Hilton Whittle, John D. Groopman

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Abstract

Aflatoxin is implicated as a risk factor for hepatocellular carcinoma in areas of the world with a high incidence of this tumor. The present study was designed to validate the use of aflatoxin-albumin adducts in peripheral blood as a measure of individual exposure to this carcinogen. Dietary intake of aflatoxin was measured at the individual level in 20 residents of Keneba, West Kiang, The Gambia, over a 7-day period and correlated with the level of aflatoxin bound to peripheral blood albumin at the beginning and end of the study. Complementary enzyme-linked immunosorbent assay and high-performance liquid chromatography-fluorescence techniques were used to assay the aflatoxin adducts. All subjects were exposed to aflatoxin originating from several food types, with an average daily intake of 1.4 Mg/day. A significant correlation (r = 0.55; P = >0.05) was observed between the dietary intake and the level of albumin-bound aflatoxin at the end of the study. In addition, a good agreement was obtained with the two analytical techniques. A comparison of matched chronic hepatitis B surface antigen carriers with noncarriers did not reveal any difference in adduct formation for a given dietary intake of aflatoxin. These studies demonstrate the validity of aflatoxin-albumin adducts as a marker of human exposure to this carcinogen.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalCancer Epidemiology Biomarkers and Prevention
Volume1
Issue number3
StatePublished - Jan 1 1992

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ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Wild, C. P., Chapot, B., Hall, A. J., Montesano, R., Hudson, G. J., Sabbioni, G., Wogan, G. N., Whittle, H., & Groopman, J. D. (1992). Dietary Intake of Aflatoxins and the Level of Albumin-bound Aflatoxin in Peripheral Blood in The Gambia, West Africa. Cancer Epidemiology Biomarkers and Prevention, 1(3), 229-234.