Dietary bile acid supplementation improves intestinal integrity and survival in a murine model

Erin E. Perrone, Chen Chen, Shannon W. Longshore, Oneybuchi Okezie, Brad W. Warner, Chen Chih Sun, Samuel M. Alaish, Eric D. Strauch

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: In vitro supplementation of the bile salt, taurodeoxycholic acid (TDCA), has been shown to stimulate proliferation and prevent intestinal apoptosis in IEC-6 cells. We hypothesize that addition of TDCA to a rodent liquid diet will be protective against induced intestinal injury. Methods: C57Bl6 mice were fed a liquid diet with or without 50-mg/(kg d) TDCA supplementation. After 6 days, the mice were injected with lipopolysaccharide (LPS) (10 mg/kg) to induce intestinal injury. Specimens were obtained 24 hours later and evaluated for intestinal apoptosis, crypt proliferation, and villus length. A separate cohort of animals was injected with LPS (25 mg/kg) and followed 7 days for survival. Results: Mice whose diet was supplemented with TDCA had significantly increased survival. After LPS-induced injury, mice supplemented with TDCA showed decreased intestinal apoptosis by both H&E and caspase-3. They also had increased intestinal proliferation by 5-bromo-2′deoxyuridine staining and increased villus length. Conclusions: Dietary TDCA supplementation alleviates mucosal damage and improves survival after LPS-induced intestinal injury. Taurodeoxycholic acid is protective of the intestinal mucosa by increasing resistance to injury-induced apoptosis, stimulating enterocyte proliferation, and increasing villus length. Taurodeoxycholic acid supplementation also results in an increased survival benefit. Therefore, bile acid supplementation may potentially protect the intestine from injury or infection.

Original languageEnglish (US)
Pages (from-to)1256-1265
Number of pages10
JournalJournal of pediatric surgery
Volume45
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

Keywords

  • Apoptosis
  • Bile salts
  • Intestinal injury
  • Proliferation
  • Taurodeoxycholic acid (TDCA)

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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