TY - JOUR
T1 - Dictyostelium myosin II mechanochemistry promotes active behavior of the cortex on long time scales
AU - Girard, Kristine D.
AU - Kuo, Scot C.
AU - Robinson, Douglas H.
PY - 2006/2/14
Y1 - 2006/2/14
N2 - Cell cortices rearrange dynamically to complete cytokinesis, crawl in response to chemoattractant, build tissues, and make neuronal connections. Highly enriched in the cell cortex, actin, myosin II, and actin crosslinkers facilitate cortical movements. Because cortical behavior is the consequence of nanoscale biochemical events, it is essential to probe the cortex at this level. Here, we use high-resolution laser-based particle tracking to examine how myosin II mechanochemistry and dynacortin-mediated actin crosslinking control cortex dynamics in Dictyostelium. Consistent with its low duty ratio, myosin II does not directly drive active bead motility. Instead, myosin II and dynacortin antagonistically regulate other active processes in the living cortex.
AB - Cell cortices rearrange dynamically to complete cytokinesis, crawl in response to chemoattractant, build tissues, and make neuronal connections. Highly enriched in the cell cortex, actin, myosin II, and actin crosslinkers facilitate cortical movements. Because cortical behavior is the consequence of nanoscale biochemical events, it is essential to probe the cortex at this level. Here, we use high-resolution laser-based particle tracking to examine how myosin II mechanochemistry and dynacortin-mediated actin crosslinking control cortex dynamics in Dictyostelium. Consistent with its low duty ratio, myosin II does not directly drive active bead motility. Instead, myosin II and dynacortin antagonistically regulate other active processes in the living cortex.
KW - Cell mechanics
KW - Dynacortin
KW - Laser-based particle tracking
KW - Nanoscale
UR - http://www.scopus.com/inward/record.url?scp=33144468161&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33144468161&partnerID=8YFLogxK
U2 - 10.1073/pnas.0508819103
DO - 10.1073/pnas.0508819103
M3 - Article
C2 - 16461463
AN - SCOPUS:33144468161
SN - 0027-8424
VL - 103
SP - 2103
EP - 2108
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -