TY - JOUR
T1 - Dictyostelium huntingtin controls chemotaxis and cytokinesis through the regulation of myosin II phosphorylation
AU - Wang, Yu
AU - Steimle, Paul A.
AU - Ren, Yixin
AU - Ross, Christopher A.
AU - Robinson, Douglas N.
AU - Egelhoff, Thomas T.
AU - Sesaki, Hiromi
AU - Iijima, Miho
PY - 2011/7/1
Y1 - 2011/7/1
N2 - Abnormalities in the huntingtin protein (Htt) are associated with Huntington's disease. Despite its importance, the function of Htt is largely unknown. We show that Htt is required for normal chemotaxis and cytokinesis in Dictyostelium discoideum. Cells lacking Htt showed slower migration toward the chemoattractant cAMP and contained lower levels of cortical myosin II, which is likely due to defects in dephosphorylation of myosin II mediated by protein phosphatase 2A (PP2A). htt - cells also failed to maintain myosin II in the cortex of the cleavage furrow, generating unseparated daughter cells connected through a thin cytoplasmic bridge. Furthermore, similar to Dictyostelium htt - cells, siRNA-mediated knockdown of human HTT also decreased the PP2A activity in HeLa cells. Our data indicate that Htt regulates the phosphorylation status of myosin II during chemotaxis and cytokinesis through PP2A.
AB - Abnormalities in the huntingtin protein (Htt) are associated with Huntington's disease. Despite its importance, the function of Htt is largely unknown. We show that Htt is required for normal chemotaxis and cytokinesis in Dictyostelium discoideum. Cells lacking Htt showed slower migration toward the chemoattractant cAMP and contained lower levels of cortical myosin II, which is likely due to defects in dephosphorylation of myosin II mediated by protein phosphatase 2A (PP2A). htt - cells also failed to maintain myosin II in the cortex of the cleavage furrow, generating unseparated daughter cells connected through a thin cytoplasmic bridge. Furthermore, similar to Dictyostelium htt - cells, siRNA-mediated knockdown of human HTT also decreased the PP2A activity in HeLa cells. Our data indicate that Htt regulates the phosphorylation status of myosin II during chemotaxis and cytokinesis through PP2A.
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U2 - 10.1091/mbc.E10-11-0926
DO - 10.1091/mbc.E10-11-0926
M3 - Article
C2 - 21562226
AN - SCOPUS:79959861118
SN - 1059-1524
VL - 22
SP - 2270
EP - 2281
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 13
ER -