Dictyostelium huntingtin controls chemotaxis and cytokinesis through the regulation of myosin II phosphorylation

Yu Wang, Paul A. Steimle, Yixin Ren, Christopher A. Ross, Douglas N. Robinson, Thomas T. Egelhoff, Hiromi Sesaki, Miho Iijima

Research output: Contribution to journalArticle

Abstract

Abnormalities in the huntingtin protein (Htt) are associated with Huntington's disease. Despite its importance, the function of Htt is largely unknown. We show that Htt is required for normal chemotaxis and cytokinesis in Dictyostelium discoideum. Cells lacking Htt showed slower migration toward the chemoattractant cAMP and contained lower levels of cortical myosin II, which is likely due to defects in dephosphorylation of myosin II mediated by protein phosphatase 2A (PP2A). htt - cells also failed to maintain myosin II in the cortex of the cleavage furrow, generating unseparated daughter cells connected through a thin cytoplasmic bridge. Furthermore, similar to Dictyostelium htt - cells, siRNA-mediated knockdown of human HTT also decreased the PP2A activity in HeLa cells. Our data indicate that Htt regulates the phosphorylation status of myosin II during chemotaxis and cytokinesis through PP2A.

Original languageEnglish (US)
Pages (from-to)2270-2281
Number of pages12
JournalMolecular biology of the cell
Volume22
Issue number13
DOIs
StatePublished - Jul 1 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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