DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration

Hiroki Kaneko, Sami Dridi, Valeria Tarallo, Bradley D. Gelfand, Benjamin J. Fowler, Won Gil Cho, Mark E. Kleinman, Steven L. Ponicsan, William W. Hauswirth, Vince A. Chiodo, Katalin Karikó, Jae Wook Yoo, Dong Ki Lee, Majda Hadziahmetovic, Ying Song, Smita Misra, Gautam Chaudhuri, Frank W. Buaas, Robert E. Braun, David R. Hinton & 22 others Qing Zhang, Hans E. Grossniklaus, Jan M. Provis, Michele C. Madigan, Ann H. Milam, Nikki L. Justice, Romulo J C Albuquerque, Alexander D. Blandford, Sasha Bogdanovich, Yoshio Hirano, Jassir Witta, Elaine Fuchs, Dan R. Littman, Balamurali K. Ambati, Charles M. Rudin, Mark M W Chong, Patrick Provost, Jennifer F. Kugel, James A. Goodrich, Joshua L. Dunaief, Judit Z. Baffi, Jayakrishna Ambati

Research output: Contribution to journalArticle

Abstract

Geographic atrophy (GA), an untreatable advanced form of age-related macular degeneration, results from retinal pigmented epithelium (RPE) cell degeneration. Here we show that the microRNA (miRNA)-processing enzyme DICER1 is reduced in the RPE of humans with GA, and that conditional ablation of Dicer1, but not seven other miRNA-processing enzymes, induces RPE degeneration in mice. DICER1 knockdown induces accumulation of Alu RNA in human RPE cells and Alu-like B1 and B2 RNAs in mouse RPE. Alu RNA is increased in the RPE of humans with GA, and this pathogenic RNA induces human RPE cytotoxicity and RPE degeneration in mice. Antisense oligonucleotides targeting Alu/B1/B2 RNAs prevent DICER1 depletion-induced RPE degeneration despite global miRNA downregulation. DICER1 degrades Alu RNA, and this digested Alu RNA cannot induce RPE degeneration in mice. These findings reveal a miRNA-independent cell survival function for DICER1 involving retrotransposon transcript degradation, show that Alu RNA can directly cause human pathology, and identify new targets for a major cause of blindness.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalNature
Volume471
Issue number7338
DOIs
StatePublished - Mar 17 2011

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Macular Degeneration
Epithelium
RNA
Geographic Atrophy
MicroRNAs
Retroelements
Antisense Oligonucleotides
Enzymes
Blindness
Cell Survival
Down-Regulation
Pathology

ASJC Scopus subject areas

  • General

Cite this

Kaneko, H., Dridi, S., Tarallo, V., Gelfand, B. D., Fowler, B. J., Cho, W. G., ... Ambati, J. (2011). DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature, 471(7338), 325-332. https://doi.org/10.1038/nature09830

DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. / Kaneko, Hiroki; Dridi, Sami; Tarallo, Valeria; Gelfand, Bradley D.; Fowler, Benjamin J.; Cho, Won Gil; Kleinman, Mark E.; Ponicsan, Steven L.; Hauswirth, William W.; Chiodo, Vince A.; Karikó, Katalin; Yoo, Jae Wook; Lee, Dong Ki; Hadziahmetovic, Majda; Song, Ying; Misra, Smita; Chaudhuri, Gautam; Buaas, Frank W.; Braun, Robert E.; Hinton, David R.; Zhang, Qing; Grossniklaus, Hans E.; Provis, Jan M.; Madigan, Michele C.; Milam, Ann H.; Justice, Nikki L.; Albuquerque, Romulo J C; Blandford, Alexander D.; Bogdanovich, Sasha; Hirano, Yoshio; Witta, Jassir; Fuchs, Elaine; Littman, Dan R.; Ambati, Balamurali K.; Rudin, Charles M.; Chong, Mark M W; Provost, Patrick; Kugel, Jennifer F.; Goodrich, James A.; Dunaief, Joshua L.; Baffi, Judit Z.; Ambati, Jayakrishna.

In: Nature, Vol. 471, No. 7338, 17.03.2011, p. 325-332.

Research output: Contribution to journalArticle

Kaneko, H, Dridi, S, Tarallo, V, Gelfand, BD, Fowler, BJ, Cho, WG, Kleinman, ME, Ponicsan, SL, Hauswirth, WW, Chiodo, VA, Karikó, K, Yoo, JW, Lee, DK, Hadziahmetovic, M, Song, Y, Misra, S, Chaudhuri, G, Buaas, FW, Braun, RE, Hinton, DR, Zhang, Q, Grossniklaus, HE, Provis, JM, Madigan, MC, Milam, AH, Justice, NL, Albuquerque, RJC, Blandford, AD, Bogdanovich, S, Hirano, Y, Witta, J, Fuchs, E, Littman, DR, Ambati, BK, Rudin, CM, Chong, MMW, Provost, P, Kugel, JF, Goodrich, JA, Dunaief, JL, Baffi, JZ & Ambati, J 2011, 'DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration', Nature, vol. 471, no. 7338, pp. 325-332. https://doi.org/10.1038/nature09830
Kaneko H, Dridi S, Tarallo V, Gelfand BD, Fowler BJ, Cho WG et al. DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature. 2011 Mar 17;471(7338):325-332. https://doi.org/10.1038/nature09830
Kaneko, Hiroki ; Dridi, Sami ; Tarallo, Valeria ; Gelfand, Bradley D. ; Fowler, Benjamin J. ; Cho, Won Gil ; Kleinman, Mark E. ; Ponicsan, Steven L. ; Hauswirth, William W. ; Chiodo, Vince A. ; Karikó, Katalin ; Yoo, Jae Wook ; Lee, Dong Ki ; Hadziahmetovic, Majda ; Song, Ying ; Misra, Smita ; Chaudhuri, Gautam ; Buaas, Frank W. ; Braun, Robert E. ; Hinton, David R. ; Zhang, Qing ; Grossniklaus, Hans E. ; Provis, Jan M. ; Madigan, Michele C. ; Milam, Ann H. ; Justice, Nikki L. ; Albuquerque, Romulo J C ; Blandford, Alexander D. ; Bogdanovich, Sasha ; Hirano, Yoshio ; Witta, Jassir ; Fuchs, Elaine ; Littman, Dan R. ; Ambati, Balamurali K. ; Rudin, Charles M. ; Chong, Mark M W ; Provost, Patrick ; Kugel, Jennifer F. ; Goodrich, James A. ; Dunaief, Joshua L. ; Baffi, Judit Z. ; Ambati, Jayakrishna. / DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. In: Nature. 2011 ; Vol. 471, No. 7338. pp. 325-332.
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abstract = "Geographic atrophy (GA), an untreatable advanced form of age-related macular degeneration, results from retinal pigmented epithelium (RPE) cell degeneration. Here we show that the microRNA (miRNA)-processing enzyme DICER1 is reduced in the RPE of humans with GA, and that conditional ablation of Dicer1, but not seven other miRNA-processing enzymes, induces RPE degeneration in mice. DICER1 knockdown induces accumulation of Alu RNA in human RPE cells and Alu-like B1 and B2 RNAs in mouse RPE. Alu RNA is increased in the RPE of humans with GA, and this pathogenic RNA induces human RPE cytotoxicity and RPE degeneration in mice. Antisense oligonucleotides targeting Alu/B1/B2 RNAs prevent DICER1 depletion-induced RPE degeneration despite global miRNA downregulation. DICER1 degrades Alu RNA, and this digested Alu RNA cannot induce RPE degeneration in mice. These findings reveal a miRNA-independent cell survival function for DICER1 involving retrotransposon transcript degradation, show that Alu RNA can directly cause human pathology, and identify new targets for a major cause of blindness.",
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AU - Kaneko, Hiroki

AU - Dridi, Sami

AU - Tarallo, Valeria

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AU - Fowler, Benjamin J.

AU - Cho, Won Gil

AU - Kleinman, Mark E.

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AU - Hinton, David R.

AU - Zhang, Qing

AU - Grossniklaus, Hans E.

AU - Provis, Jan M.

AU - Madigan, Michele C.

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AU - Albuquerque, Romulo J C

AU - Blandford, Alexander D.

AU - Bogdanovich, Sasha

AU - Hirano, Yoshio

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AU - Rudin, Charles M.

AU - Chong, Mark M W

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