Diagnostic significance of allelic imbalance in cancer

Christopher M. Heaphy, Marco Bisoffi, Jeffrey K. Griffith

Research output: Contribution to journalReview articlepeer-review

Abstract

Allelic imbalance (AI), a deviation from the normal 1:1 ratio of maternal and paternal alleles, occurs in virtually all solid and blood-borne malignancies. The frequency and spectrum of AI in a tumor cell reflects the karyotypic complexity of the cancer genome. Hence, many investigations have assessed the extent of AI to analyze differences between normal and tumor tissues in a variety of different organs. In this review, the authors describe established and emerging technologies used to assess the extent of AI in human tissues, and their application in the diagnosis of cancer. The four major methods to be reviewed represent powerful and widely used tools for the identification of allelic imbalances accompanying cancer initiation and progression. These are fluorescent in situ hybridization, comparative genomic hybridization, single nucleotide polymorphism arrays and the use of microsatellite markers. For each method, the authors provide a brief description of the approach and elaborate on specific studies that highlight its utility in the diagnosis of human cancers.

Original languageEnglish (US)
Pages (from-to)159-168
Number of pages10
JournalExpert Opinion on Medical Diagnostics
Volume1
Issue number2
DOIs
StatePublished - Oct 2007
Externally publishedYes

Keywords

  • CGH
  • FISH
  • SNP
  • allelic imbalance
  • cancer
  • microsatellite markers

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Biochemistry, medical

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