Diagnostic performance of placental alpha-microglobulin-1 test in women with prolonged pre-labour rupture of membranes

George Uchenna Eleje, Euzebus Chinonye Ezugwu, Ahizechukwu Eke, Lydia Ijeoma Eleje, Joseph Ifeanyichukwu Ikechebelu, Evaristus Anthony Afiadigwe, Frank O. Ezugwu, Gerald Okanandu Udigwe, Charles I. Okafor, Chukwuemeka Okwudili Ezeama

Research output: Contribution to journalArticle

Abstract

Objectives: To determine diagnostic performance of placental alpha-microglobulin-1 (PAMG-1) test compared to conventional clinical assessment (CCA) in women with prolonged pre-labour rupture of membranes (PROM).Methods: A double-blind study of women with symptoms and signs of PROM in Nnamdi Azikiwe University Teaching Hospital, Nnewi and University of Nigeria Teaching Hospital, Enugu, in south-east Nigeria using CCA for PROM and PAMG-1 test was done. Women were included if their symptoms, signs or complaints suggestive of PROM was more than 24 h duration. PROM was diagnosed if two out of three methods from CCA (pooling, positive nitrazine test or ferning) were present. Confirmation of PROM was done after delivery using any two of these clinical criteria: delivery in 48 h to 7 days, evidence of chorioamnionitis, membranes obviously ruptured at delivery and adverse perinatal outcomes strongly correlated with prolonged PROM.Results: Accuracy, specificity and sensitivity value for CCA were 72.5, 36.8 and 86.0% lower than for PAMG-1 test which were 95.7, 94.1 and 96.2%. In equivocal cases, PAMG-1 was significantly more accurate than CCA (92.3% versus 38.5%; p < 0.001).Conclusions: This study in women with prolonged PROM, confirms that PAMG-1 test has high diagnostic accuracy irrespective of the duration of PROM before clinical evaluation.

Original languageEnglish (US)
Pages (from-to)1291-1296
Number of pages6
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume29
Issue number8
DOIs
StatePublished - Apr 17 2016
Externally publishedYes

Fingerprint

Rupture
Membranes
Nigeria
Teaching Hospitals
Signs and Symptoms
alpha-1-microglobulin
Chorioamnionitis
Double-Blind Method
Sensitivity and Specificity

Keywords

  • Accuracy
  • conventional clinical assessment
  • PAMG-1
  • prolonged rupture of membranes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Diagnostic performance of placental alpha-microglobulin-1 test in women with prolonged pre-labour rupture of membranes. / Eleje, George Uchenna; Ezugwu, Euzebus Chinonye; Eke, Ahizechukwu; Eleje, Lydia Ijeoma; Ikechebelu, Joseph Ifeanyichukwu; Afiadigwe, Evaristus Anthony; Ezugwu, Frank O.; Udigwe, Gerald Okanandu; Okafor, Charles I.; Ezeama, Chukwuemeka Okwudili.

In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 29, No. 8, 17.04.2016, p. 1291-1296.

Research output: Contribution to journalArticle

Eleje, GU, Ezugwu, EC, Eke, A, Eleje, LI, Ikechebelu, JI, Afiadigwe, EA, Ezugwu, FO, Udigwe, GO, Okafor, CI & Ezeama, CO 2016, 'Diagnostic performance of placental alpha-microglobulin-1 test in women with prolonged pre-labour rupture of membranes', Journal of Maternal-Fetal and Neonatal Medicine, vol. 29, no. 8, pp. 1291-1296. https://doi.org/10.3109/14767058.2015.1046375
Eleje, George Uchenna ; Ezugwu, Euzebus Chinonye ; Eke, Ahizechukwu ; Eleje, Lydia Ijeoma ; Ikechebelu, Joseph Ifeanyichukwu ; Afiadigwe, Evaristus Anthony ; Ezugwu, Frank O. ; Udigwe, Gerald Okanandu ; Okafor, Charles I. ; Ezeama, Chukwuemeka Okwudili. / Diagnostic performance of placental alpha-microglobulin-1 test in women with prolonged pre-labour rupture of membranes. In: Journal of Maternal-Fetal and Neonatal Medicine. 2016 ; Vol. 29, No. 8. pp. 1291-1296.
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AU - Eleje, Lydia Ijeoma

AU - Ikechebelu, Joseph Ifeanyichukwu

AU - Afiadigwe, Evaristus Anthony

AU - Ezugwu, Frank O.

AU - Udigwe, Gerald Okanandu

AU - Okafor, Charles I.

AU - Ezeama, Chukwuemeka Okwudili

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N2 - Objectives: To determine diagnostic performance of placental alpha-microglobulin-1 (PAMG-1) test compared to conventional clinical assessment (CCA) in women with prolonged pre-labour rupture of membranes (PROM).Methods: A double-blind study of women with symptoms and signs of PROM in Nnamdi Azikiwe University Teaching Hospital, Nnewi and University of Nigeria Teaching Hospital, Enugu, in south-east Nigeria using CCA for PROM and PAMG-1 test was done. Women were included if their symptoms, signs or complaints suggestive of PROM was more than 24 h duration. PROM was diagnosed if two out of three methods from CCA (pooling, positive nitrazine test or ferning) were present. Confirmation of PROM was done after delivery using any two of these clinical criteria: delivery in 48 h to 7 days, evidence of chorioamnionitis, membranes obviously ruptured at delivery and adverse perinatal outcomes strongly correlated with prolonged PROM.Results: Accuracy, specificity and sensitivity value for CCA were 72.5, 36.8 and 86.0% lower than for PAMG-1 test which were 95.7, 94.1 and 96.2%. In equivocal cases, PAMG-1 was significantly more accurate than CCA (92.3% versus 38.5%; p < 0.001).Conclusions: This study in women with prolonged PROM, confirms that PAMG-1 test has high diagnostic accuracy irrespective of the duration of PROM before clinical evaluation.

AB - Objectives: To determine diagnostic performance of placental alpha-microglobulin-1 (PAMG-1) test compared to conventional clinical assessment (CCA) in women with prolonged pre-labour rupture of membranes (PROM).Methods: A double-blind study of women with symptoms and signs of PROM in Nnamdi Azikiwe University Teaching Hospital, Nnewi and University of Nigeria Teaching Hospital, Enugu, in south-east Nigeria using CCA for PROM and PAMG-1 test was done. Women were included if their symptoms, signs or complaints suggestive of PROM was more than 24 h duration. PROM was diagnosed if two out of three methods from CCA (pooling, positive nitrazine test or ferning) were present. Confirmation of PROM was done after delivery using any two of these clinical criteria: delivery in 48 h to 7 days, evidence of chorioamnionitis, membranes obviously ruptured at delivery and adverse perinatal outcomes strongly correlated with prolonged PROM.Results: Accuracy, specificity and sensitivity value for CCA were 72.5, 36.8 and 86.0% lower than for PAMG-1 test which were 95.7, 94.1 and 96.2%. In equivocal cases, PAMG-1 was significantly more accurate than CCA (92.3% versus 38.5%; p < 0.001).Conclusions: This study in women with prolonged PROM, confirms that PAMG-1 test has high diagnostic accuracy irrespective of the duration of PROM before clinical evaluation.

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KW - conventional clinical assessment

KW - PAMG-1

KW - prolonged rupture of membranes

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