Diagnosis of Schistosoma haematobium by detection of specific DNA fragments from filtered urine samples

Olufunmilola A. Ibironke, Anna E. Phillips, Amadou Garba, Sani M. Lamine, Clive Shiff

Research output: Contribution to journalArticlepeer-review

Abstract

Definitive diagnosis of Schistosoma haematobium infection in adult patients is a clinically important challenge. Chronically infected adults pass few eggs in the urine, which are often missed when current diagnostic methods are used. In the work presented here, we report on an alternative diagnostic method based on presence of the S. haematobium-specific Dra 1, 121 bp repeat fragment in human urine. A novel method of collecting the urine specimens in the field and filtering them through heavy Whatman No. 3 paper was introduced. After drying, the samples remained viable for several months at room temperature. To test the potential use of this method, 89 urine specimens from school children in Kollo District, Niger, were examined. In all, 52 of 89 (58.4%) were positive for hematuria, 4 of 89 (49.4%) were positive for eggs, and 51 of 89 (57.3%) showed parasite-specific DNA. These were compared with 60 filtered urine specimens obtained from random samples of adults from two study sites in Nigeria, one endemic and one non-endemic for S. haematobium. In the 30 patients from the endemic site, all 10 samples with detectable eggs and 7 of the 20 egg-negative samples were DNA positive. It was concluded that the urine filter paper method was sufficiently sensitive to detect low and cryptic infections, that DNA detection was more sensitive than egg detection, and that the filtration method facilitated specimen collection and transport from the field.

Original languageEnglish (US)
Pages (from-to)998-1001
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume84
Issue number6
DOIs
StatePublished - Jun 2011

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Diagnosis of Schistosoma haematobium by detection of specific DNA fragments from filtered urine samples'. Together they form a unique fingerprint.

Cite this