Diagnosis of human immunodeficiency virus infection by immunoassay using a molecularly cloned and expressed virus envelope polypeptide. Comparison to Western blot on 2707 consecutive serum samples

D. S. Burke, B. L. Brandt, R. R. Redfield, T. H. Lee, R. M. Thorn, G. A. Beltz, C. H. Hung

Research output: Contribution to journalArticle

Abstract

To detect human immunodeficiency virus (HIV) antibodies in a simple enzyme-linked immunoassay (CBr3-EIA), we used an Escherichia coli-expressed polypeptide antigen, representing the carboxy-terminal third of the external membrane glycoprotein gene fused with the aminoterminal half of the transmembrane glycoprotein gene. Over a 3-month period, 2707 consecutive serum samples referred for confirmatory testing for human T-lymphotrophic virus type III (HTLV-III) antibodies were evaluated by both Western blot and CBre3-EIA. On a single determination for each sample, the CBre3-EIA was found to have an estimated sensitivity (99.9%) and specificity (99.1%) similar or superior to the more cumbersome Western blot method. This study shows that all HIV-seropositive subjects have antibodies to the virus envelope protein; no other virus antigens are required for construction of highly sensitive immunoassays.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalAnnals of Internal Medicine
Volume106
Issue number5
StatePublished - 1987
Externally publishedYes

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Virus Diseases
Immunoassay
Western Blotting
HIV
Viruses
Peptides
Antibodies
Serum
Viral Envelope Proteins
Antigens
Membrane Glycoproteins
Immunoenzyme Techniques
Genes
Glycoproteins
Escherichia coli
Sensitivity and Specificity

ASJC Scopus subject areas

  • Medicine(all)

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Diagnosis of human immunodeficiency virus infection by immunoassay using a molecularly cloned and expressed virus envelope polypeptide. Comparison to Western blot on 2707 consecutive serum samples. / Burke, D. S.; Brandt, B. L.; Redfield, R. R.; Lee, T. H.; Thorn, R. M.; Beltz, G. A.; Hung, C. H.

In: Annals of Internal Medicine, Vol. 106, No. 5, 1987, p. 671-676.

Research output: Contribution to journalArticle

Burke, D. S. ; Brandt, B. L. ; Redfield, R. R. ; Lee, T. H. ; Thorn, R. M. ; Beltz, G. A. ; Hung, C. H. / Diagnosis of human immunodeficiency virus infection by immunoassay using a molecularly cloned and expressed virus envelope polypeptide. Comparison to Western blot on 2707 consecutive serum samples. In: Annals of Internal Medicine. 1987 ; Vol. 106, No. 5. pp. 671-676.
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AU - Hung, C. H.

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